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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleNon-inclusion Complexes Between Riboflavin And Cyclodextrins.pt_BR
dc.contributor.authorde Jesus, Marcelo Bispopt_BR
dc.contributor.authorFraceto, Leonardo Fernandespt_BR
dc.contributor.authorMartini, Maria Florenciapt_BR
dc.contributor.authorPickholz, Monicapt_BR
dc.contributor.authorFerreira, Carmen Veríssimapt_BR
dc.contributor.authorde Paula, Eneidapt_BR
unicamp.authorMarcelo Bispo de Jesus, Department of Biochemistry, Institute of Biology, State University of Campinas-Unicamp, Campinas, Brazil.pt_BR
unicamp.author.externalLeonardo Fernandes Fraceto,pt
unicamp.author.externalMaria Florencia Martini,pt
unicamp.author.externalMonica Pickholz,pt
unicamp.author.externalCarmen Veríssima Ferreira,pt
unicamp.author.externalEneida de Paula,pt
dc.subjectAntineoplastic Agentspt_BR
dc.subjectCell Line, Tumorpt_BR
dc.subjectDrug Carrierspt_BR
dc.subjectHumanspt_BR
dc.subjectHydrogen Bondingpt_BR
dc.subjectMalept_BR
dc.subjectProstatic Neoplasmspt_BR
dc.subjectRiboflavinpt_BR
dc.subjectSolubilitypt_BR
dc.subjectBeta-cyclodextrinspt_BR
dc.description.abstractTo investigate the molecular interaction between β-cyclodextrin (βCD) or hydroxypropyl-β-cyclodextrin (HPβCD) and riboflavin (RF), and to test the anticancer potential of these formulations. The physicochemical characterization of the association between RF and CDs was performed by UV-vis absorption, fluorescence, differential scanning calorimetry and NMR techniques. Molecular dynamics simulation was used to shed light on the mechanism of interaction of RF and CDs. Additionally, in-vitro cell culture tests were performed to evaluate the cytotoxicity of the RF-CD complexes against prostate cancer cells. Neither βCD nor HPβCD led to substantial changes in the physicochemical properties of RF (with the exception of solubility). Additionally, rotating frame Overhauser effect spectroscopy experiments detected no spatial correlations between hydrogens from the internal cavity of CDs and RF, while molecular dynamics simulations revealed 'out-of-ring' RF-CD interactions. Notwithstanding, both RF-βCD and RF-HPβCD complexes were cytotoxic to PC3 prostate cancer cells. The interaction between RF and either βCD or HPβCD, at low concentrations, seems to be made through hydrogen bonding between the flavonoid and the external rim of both CDs. Regardless of the mechanism of complexation, our findings indicate that RF-CD complexes significantly increase RF solubility and potentiate its antitumour effect.en
dc.relation.ispartofThe Journal Of Pharmacy And Pharmacologypt_BR
dc.relation.ispartofabbreviationJ. Pharm. Pharmacol.pt_BR
dc.date.issued2012-Junpt_BR
dc.identifier.citationThe Journal Of Pharmacy And Pharmacology. v. 64, n. 6, p. 832-42, 2012-Jun.pt_BR
dc.language.isoengpt_BR
dc.description.volume64pt_BR
dc.description.firstpage832-42pt_BR
dc.rightsfechadopt_BR
dc.rights.holder© 2012 The Authors. JPP © 2012 Royal Pharmaceutical Society.pt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn2042-7158pt_BR
dc.identifier.doi10.1111/j.2042-7158.2012.01492.xpt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/22571261pt_BR
dc.date.available2015-11-27T13:28:30Z-
dc.date.accessioned2015-11-27T13:28:30Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:28:30Z (GMT). No. of bitstreams: 1 pmed_22571261.pdf: 1278079 bytes, checksum: 3877519bf24314886e74630dcbb94542 (MD5) Previous issue date: 2012en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200000-
dc.identifier.idPubmed22571261pt_BR
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