Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199997
Type: Artigo de periódico
Title: Quantification Of Cultivable Bacteria And Endotoxin In Post-treatment Apical Periodontitis Before And After Chemo-mechanical Preparation.
Author: Endo, M S
Martinho, F C
Zaia, A A
Ferraz, C C R
Almeida, J F A
Gomes, B P F A
Abstract: This clinical study was conducted to quantify cultivable bacteria and endotoxin in root canals with post-treatment apical periodontitis by correlating their levels with clinical features and to evaluate the effect of chemo-mechanical preparation (CMP) with 2 % chlorhexidine gel + 17 % EDTA on bacterial and endotoxin removal/elimination. Moreover, target strict Gram-negative anaerobic bacteria were investigated by polymerase chain reaction (PCR). Fifteen teeth with post-treatment apical periodontitis were sampled before (s1) and after (s2) CMP. Culture techniques determined the number of colony-forming units (CFU). PCR (16S rDNA) and limulus amebocyte lysate (LAL) assay were used for bacterial and endotoxin detection, respectively. Prevotella nigrescens (4/15), Prevotella intermedia (2/15), and Tannerella forsythia (2/15) were the most frequently detected species. Endotoxin was recovered in 100 % of the samples. At s1, bacteria and endotoxin were detected at a median value of 5.14 × 10(3) CFU/mL and 3.96 EU/mL, respectively. Higher levels of endotoxin were related to a larger size of radiolucent area (>5 mm) (p < 0.05). CMP was more effective in reducing bacteria (99.61 %) than endotoxin (60.6 %) (both p < 0.05). Our findings indicated that the levels of endotoxin found in infected root canals were related to a larger size of radiolucent area in the periapical region. Moreover, CMP was effective in reducing both bacterial and endotoxin contents in post-treatment apical periodontitis.
Subject: Anti-bacterial Agents
Bacterial Load
Bacterial Typing Techniques
Chlorhexidine
Dental Pulp Cavity
Edetic Acid
Endotoxins
Humans
Microbial Viability
Periapical Periodontitis
Polymerase Chain Reaction
Prevotella
Rna, Bacterial
Rna, Ribosomal, 16s
Treatment Outcome
Treponema
Rights: fechado
Identifier DOI: 10.1007/s10096-012-1598-6
Address: http://www.ncbi.nlm.nih.gov/pubmed/22565224
Date Issue: 2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

Files in This Item:
File SizeFormat 
pmed_22565224.pdf182.53 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.