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Type: Artigo de periódico
Title: Quantification Of Cultivable Bacteria And Endotoxin In Post-treatment Apical Periodontitis Before And After Chemo-mechanical Preparation.
Author: Endo, M S
Martinho, F C
Zaia, A A
Ferraz, C C R
Almeida, J F A
Gomes, B P F A
Abstract: This clinical study was conducted to quantify cultivable bacteria and endotoxin in root canals with post-treatment apical periodontitis by correlating their levels with clinical features and to evaluate the effect of chemo-mechanical preparation (CMP) with 2 % chlorhexidine gel + 17 % EDTA on bacterial and endotoxin removal/elimination. Moreover, target strict Gram-negative anaerobic bacteria were investigated by polymerase chain reaction (PCR). Fifteen teeth with post-treatment apical periodontitis were sampled before (s1) and after (s2) CMP. Culture techniques determined the number of colony-forming units (CFU). PCR (16S rDNA) and limulus amebocyte lysate (LAL) assay were used for bacterial and endotoxin detection, respectively. Prevotella nigrescens (4/15), Prevotella intermedia (2/15), and Tannerella forsythia (2/15) were the most frequently detected species. Endotoxin was recovered in 100 % of the samples. At s1, bacteria and endotoxin were detected at a median value of 5.14 × 10(3) CFU/mL and 3.96 EU/mL, respectively. Higher levels of endotoxin were related to a larger size of radiolucent area (>5 mm) (p < 0.05). CMP was more effective in reducing bacteria (99.61 %) than endotoxin (60.6 %) (both p < 0.05). Our findings indicated that the levels of endotoxin found in infected root canals were related to a larger size of radiolucent area in the periapical region. Moreover, CMP was effective in reducing both bacterial and endotoxin contents in post-treatment apical periodontitis.
Subject: Anti-bacterial Agents
Bacterial Load
Bacterial Typing Techniques
Dental Pulp Cavity
Edetic Acid
Microbial Viability
Periapical Periodontitis
Polymerase Chain Reaction
Rna, Bacterial
Rna, Ribosomal, 16s
Treatment Outcome
Rights: fechado
Identifier DOI: 10.1007/s10096-012-1598-6
Date Issue: 2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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