Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199989
Type: Artigo de periódico
Title: Enzymatic Activity And Proteomic Profile Of Class Iii Peroxidases During Sugarcane Stem Development.
Author: Cesarino, Igor
Araújo, Pedro
Sampaio Mayer, Juliana Lischka
Paes Leme, Adriana Franco
Mazzafera, Paulo
Abstract: Class III peroxidases are present as large multigene families in all land plants. This large number of genes together with the diversity of processes catalyzed by peroxidases suggests possible functional specialization of each isoform. However, assigning a precise role for each individual peroxidase gene has continued to be a major bottleneck. Here we investigated the enzyme activity and translational profile of class III peroxidases during stem development of sugarcane as a first step in the estimation of physiological functions of individual isoenzymes. Internodes at three different developmental stages (young, developing and mature) were divided into pith (inner tissue) and rind (outer tissue) fractions. The rind of mature internodes presented the highest enzymatic activity and thus could be considered the ideal tissue for the discovery of peroxidase gene function. In addition, activity staining of 2DE gels revealed different isoperoxidase profiles and protein expression regulation among different tissue fractions. In-gel tryptic digestion of excised spots followed by peptide sequencing by LC-MS/MS positively matched uncharacterized peroxidases in the sugarcane database SUCEST. Multiple spots matching the same peroxidase gene were found, which reflects the generation of more than one isoform from a particular gene by post-translational modifications. The identified sugarcane peroxidases appear to be monocot-specific sequences with no clear ortholog in dicot model plant Arabidopsis thaliana.
Subject: Electrophoresis, Gel, Two-dimensional
Isoenzymes
Mass Spectrometry
Peroxidases
Phylogeny
Plant Proteins
Plant Stems
Protein Biosynthesis
Proteome
Proteomics
Saccharum
Time Factors
Rights: fechado
Identifier DOI: 10.1016/j.plaphy.2012.03.014
Address: http://www.ncbi.nlm.nih.gov/pubmed/22551762
Date Issue: 2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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