Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199955
Type: Artigo de periódico
Title: Expression Pattern Of Plunc Proteins As An Auxiliary Tool For The Diagnosis Of High-grade Mucoepidermoid Carcinoma Of The Salivary Gland.
Author: González-Arriagada, Wilfredo A
Santos-Silva, Alan R
Ito, Fabio A
Vargas, Pablo A
Speight, Paul M
Bingle, Lynne
Lopes, Marcio A
Abstract: Mucoepidermoid carcinomas are the most frequent malignant neoplasia of the salivary glands and are histologically classified as low, intermediate, and high grade. At present, histochemical stains such as periodic acid-Schiff or mucicarmine are useful tools in making a diagnosis. Recently, expression of the PLUNC proteins has been described in mucin-producing salivary gland tumors, with the suggestion that they could provide a powerful tool for the diagnosis of difficult cases. This study evaluates the expression of PLUNC proteins in 30 cases of salivary gland mucoepidermoid carcinomas. Tumors were reviewed and classified according to histological grade. Periodic acid-Schiff, mucicarmine, and immunohistochemical staining for SPLUNC1, LPLUNC1, SPLUNC2, and LPLUNC2 were carried out. Immunostaining was classified as positive or negative. The majority of the tumors (63%) were classified as low grade, 13% were intermediate grade, and 23% were high grade. SPLUNC1 (90%) and LPLUNC1 (93%) were positive in the majority of cases, mainly in mucous cells, mucin plugs, and intermediate cells. SPLUNC2 and LPLUNC2 did not present significative expression within the tumors; however, LPLUNC2 was found to stain positively in mast cells in 83% of the samples. SPLUNC1 and LPLUNC1 showed a similar pattern of expression and could prove useful in the diagnosis of high-grade cases because of the differential staining in intermediate and epidermoid cells. The expression of LPLUNC2 in mast cells has not previously been reported, but further studies are necessary to validate this finding and to determine its significance.
Subject: Adolescent
Adult
Aged
Aged, 80 And Over
Autoantigens
Carcinoma, Mucoepidermoid
Carmine
Child
Child, Preschool
Female
Gene Expression Regulation, Neoplastic
Glycoproteins
Humans
Immunohistochemistry
Leucine Zippers
Male
Mast Cells
Middle Aged
Mucins
Mucous Membrane
Neoplasm Grading
Phosphoproteins
Proteins
Retrospective Studies
Salivary Gland Neoplasms
Salivary Glands, Minor
Salivary Proteins And Peptides
Tumor Markers, Biological
Young Adult
Rights: fechado
Identifier DOI: 10.1111/j.1600-0714.2012.01145.x
Address: http://www.ncbi.nlm.nih.gov/pubmed/22487508
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_22487508.pdf1.16 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.