Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199936
Type: Artigo de periódico
Title: Analysis Of The Physical Activity Effects And Measurement Of Pro-inflammatory Cytokines In Irradiated Lungs In Rats.
Author: Bianchi, Renata Cristiane Gennari
Ropelle, Eduardo Rochete
Katashima, Carlos Kiyoshi
Carvalheira, José Barreto Campello
Lopes, Luiz Roberto
Andreollo, Nelson Adami
Abstract: To study if the pre-radiotherapy physical activity has radio-protective elements, by measuring the radio-induced activation of pro-inflammatory cytokines as interleukin-6 (il-6), transforming growth factor -β (tgf -β), tumor necrosis factor -α (tnf-α) and protein beta kinase β (ikkβ), through western blotting analysis. A randomized study with 28 Wistar hannover rats, males, with a mean age of 90 days and weighing about 200 grams. The animals were divided into three groups: (GI, GII and GIII). GIII group were submitted to swimming for eight weeks (zero load, three times a week, about 30 minutes). Then, the groups (except the control group) were submitted to irradiation by cobalt therapy, single dose of 3.5 gray in the whole body. All animals were sacrificed by overdose of pentobarbital, according to the time for analysis of cytokines, and then a fragment of the lower lobe of the right lung went to western blotting analysis. The cytokines IKK β, TNF-α and IL-6 induced by radiation in the lung were lower in the exercised animals. However, exercise did not alter the radiation-induced increase in tgf-β. The results show a lower response in relation to inflammatory cytokines in the group that practiced the exercise pre-radiotherapy, showing that exercise can protect tissues from tissue damage due to irradiation.
Subject: Analysis Of Variance
Animals
Blotting, Western
Cobalt Radioisotopes
I-kappa B Kinase
Interleukin-6
Lung
Male
Physical Conditioning, Animal
Radiation Injuries, Experimental
Random Allocation
Rats
Rats, Wistar
Time Factors
Tumor Necrosis Factor-alpha
Rights: aberto
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/22460252
Date Issue: 2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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