Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199929
Type: Artigo de periódico
Title: Evaluation Of Cytotoxicity And Up-regulation Of Gelatinases In Fibroblast Cells By Three Root Repair Materials.
Author: Silva, E J N L
Herrera, D R
Almeida, J F A
Ferraz, C C R
Gomes, B P F A
Zaia, A A
Abstract: To investigate the effects of root repair materials on the cytotoxicity and gelatinolytic activity of matrix metalloproteinases (MMPs) in 3T3 fibroblasts. Fibroblasts (3T3, 3 × 10(5) cells per well) were incubated with elutes of calcium hydroxide (Biodinâmica, Ibiporã, PR, Brazil), EndoBinder (Binderware, São Carlos, SP, Brazil) and mineral trioxide aggregate (MTA) (Angelus, Londrina, PR, Brazil) for 24 h. The cytotoxicity of all root repair materials was determined using the MTT assay. Supernatants of cell cultures incubated with materials were collected after 24 h to determine the levels of MMP-2 and MMP-9 gelatinolytic activity by gelatin zymography. Data were analysed using anova and Tukey's test. Cells secreted MMP-2 after 24 h with calcium hydroxide inducing significantly greater MMP-2 expression in relation to the control and the other root repair materials (P < 0.05). The cytotoxicity results revealed that there was no significant difference in the cell viability of MTA, EndoBinder and the control group. However, there was a significantly reduced cell viability of 3T3 fibroblasts in association with calcium hydroxide (P < 0.05). Calcium hydroxide was associated with significantly less cell viability when compared with EndoBinder and MTA. All materials had gelatinolytic activity for MMP-2 with calcium hydroxide being associated with the greatest activity.
Subject: 3t3 Cells
Aluminum Compounds
Animals
Calcium Compounds
Calcium Hydroxide
Cell Culture Techniques
Cell Survival
Coloring Agents
Drug Combinations
Fibroblasts
Materials Testing
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Mice
Oxides
Root Canal Filling Materials
Silicates
Tetrazolium Salts
Thiazoles
Time Factors
Up-regulation
Rights: fechado
Identifier DOI: 10.1111/j.1365-2591.2012.02038.x
Address: http://www.ncbi.nlm.nih.gov/pubmed/22452531
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_22452531.pdf147.51 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.