Please use this identifier to cite or link to this item:
Type: Artigo de periódico
Title: Evaluation Of Cytotoxicity And Up-regulation Of Gelatinases In Fibroblast Cells By Three Root Repair Materials.
Author: Silva, E J N L
Herrera, D R
Almeida, J F A
Ferraz, C C R
Gomes, B P F A
Zaia, A A
Abstract: To investigate the effects of root repair materials on the cytotoxicity and gelatinolytic activity of matrix metalloproteinases (MMPs) in 3T3 fibroblasts. Fibroblasts (3T3, 3 × 10(5) cells per well) were incubated with elutes of calcium hydroxide (Biodinâmica, Ibiporã, PR, Brazil), EndoBinder (Binderware, São Carlos, SP, Brazil) and mineral trioxide aggregate (MTA) (Angelus, Londrina, PR, Brazil) for 24 h. The cytotoxicity of all root repair materials was determined using the MTT assay. Supernatants of cell cultures incubated with materials were collected after 24 h to determine the levels of MMP-2 and MMP-9 gelatinolytic activity by gelatin zymography. Data were analysed using anova and Tukey's test. Cells secreted MMP-2 after 24 h with calcium hydroxide inducing significantly greater MMP-2 expression in relation to the control and the other root repair materials (P < 0.05). The cytotoxicity results revealed that there was no significant difference in the cell viability of MTA, EndoBinder and the control group. However, there was a significantly reduced cell viability of 3T3 fibroblasts in association with calcium hydroxide (P < 0.05). Calcium hydroxide was associated with significantly less cell viability when compared with EndoBinder and MTA. All materials had gelatinolytic activity for MMP-2 with calcium hydroxide being associated with the greatest activity.
Subject: 3t3 Cells
Aluminum Compounds
Calcium Compounds
Calcium Hydroxide
Cell Culture Techniques
Cell Survival
Coloring Agents
Drug Combinations
Materials Testing
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Root Canal Filling Materials
Tetrazolium Salts
Time Factors
Rights: fechado
Identifier DOI: 10.1111/j.1365-2591.2012.02038.x
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_22452531.pdf147.51 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.