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Type: Artigo de periódico
Title: Lysine Degradation Through The Saccharopine Pathway In Bacteria: Lkr And Sdh In Bacteria And Its Relationship To The Plant And Animal Enzymes.
Author: Serrano, Guilherme Coutinho de Mello
Rezende e Silva Figueira, Thaís
Kiyota, Eduardo
Zanata, Natalia
Arruda, Paulo
Abstract: Lysine degradation through the saccharopine pathway has been shown only in plants and animals. Here, we show that bacteria possess the genes encoding lysine-ketoglutarate reductase (LKR) and saccharopine dehydrogenase (SDH). In Silicibacter, the contiguous lkr and sdh genes are interspersed, in another frame, by a polypeptide of unknown function. The bacterial enzyme does not contain the 110-amino-acid interdomain (ID) that intersperses the LKR and SDH domains of the plant enzyme. The ID was found in Cyanobacteria interspersing polypeptides without similarities and activities of LKR and SDH. The LKR/SDH bifunctional polypeptide of animals and plants may have arisen from a α-proteobacterium with a configuration similar to that of Silicibacter, whereas the ID in the plant enzyme may have been inherited from Cyanobacteria.
Subject: Amino Acid Sequence
Molecular Sequence Data
Protein Structure, Tertiary
Recombinant Proteins
Saccharopine Dehydrogenases
Sequence Alignment
Rights: fechado
Identifier DOI: 10.1016/j.febslet.2012.02.023
Date Issue: 2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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