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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleA Heat-stable Cytotoxic Factor Produced By Achromobacter Xylosoxidans Isolated From Brazilian Patients With Cf Is Associated With In Vitro Increased Proinflammatory Cytokines.pt_BR
dc.contributor.authorMantovani, Rebeca Ppt_BR
dc.contributor.authorLevy, Carlos Ept_BR
dc.contributor.authorYano, Tomomasapt_BR
unicamp.authorRebeca P Mantovani, Department of Genetics, Evolution and Bioagents, Biology Institute, State University of Campinas (UNICAMP), Campinas, SP, Brazil. rebecapmantovani@yahoo.com.brpt_BR
unicamp.author.externalCarlos E Levy,pt
unicamp.author.externalTomomasa Yano,pt
dc.subjectAchromobacter Denitrificanspt_BR
dc.subjectBrazilpt_BR
dc.subjectCarcinoma, Mucoepidermoidpt_BR
dc.subjectCell Line, Tumorpt_BR
dc.subjectCell Survivalpt_BR
dc.subjectCulture Media, Conditionedpt_BR
dc.subjectCystic Fibrosispt_BR
dc.subjectEndotoxinspt_BR
dc.subjectGram-negative Bacterial Infectionspt_BR
dc.subjectHumanspt_BR
dc.subjectIn Vitro Techniquespt_BR
dc.subjectInterleukin-6pt_BR
dc.subjectInterleukin-8pt_BR
dc.subjectPneumoniapt_BR
dc.subjectSputumpt_BR
dc.subjectVirulencept_BR
dc.description.abstractRecently, Achromobacter xylosoxidans has been related to chronic lung diseases in patients suffering from cystic fibrosis (CF), but its involvement has not been elucidated. Some virulence properties of A. xylosoxidans isolated from Brazilian patients with CF were revealed in this work. This study examined the production of a cytotoxic factor of A. xylosoxidans capable of stimulating the secretion of inflammatory cytokines (IL-6 and IL-8) from lung mucoepidermoid carcinoma cells (NCI-H292). The cytokines were measured using enzyme-linked immunosorbent (ELISA) assays. To investigate whether the cytotoxic factors may be endotoxins, they were treated with polymyxin B. The culture supernatants of all A. xylosoxidans produced a heat stable, active cytotoxin in NCI-H292 cells capable of leading to intracellular vacuoles and subsequent cell contact loss, chromatin condensation, a picnotic nucleus and cell death. There was a higher concentration of proinflammatory cytokines in the NCI-H292 cells after 24 h of incubation, with the fraction greater than 50 kDa from the culture supernatant. The cytotoxin activity remained even after treatment with polymyxin B, which suggested that the release of IL-6 and IL-8 was not stimulated by lipopolysaccharide (LPS). The cytotoxic factor produced by A. xylosoxidans may represent an important virulence factor, which when associated with CF chronic lung inflammation, may cause tissue damage and decline of lung function.en
dc.relation.ispartofJournal Of Cystic Fibrosis : Official Journal Of The European Cystic Fibrosis Societypt_BR
dc.relation.ispartofabbreviationJ. Cyst. Fibros.pt_BR
dc.date.issued2012-Julpt_BR
dc.identifier.citationJournal Of Cystic Fibrosis : Official Journal Of The European Cystic Fibrosis Society. v. 11, n. 4, p. 305-11, 2012-Jul.pt_BR
dc.language.isoengpt_BR
dc.description.volume11pt_BR
dc.description.firstpage305-11pt_BR
dc.rightsfechadopt_BR
dc.rights.holderCopyright © 2012 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.pt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1873-5010pt_BR
dc.identifier.doi10.1016/j.jcf.2012.02.002pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/22440828pt_BR
dc.date.available2015-11-27T13:28:19Z-
dc.date.accessioned2015-11-27T13:28:19Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:28:19Z (GMT). No. of bitstreams: 1 pmed_22440828.pdf: 864157 bytes, checksum: 95e0fdd2e2a0af198ccdb3c5aab31944 (MD5) Previous issue date: 2012en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199917-
dc.identifier.idPubmed22440828pt_BR
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