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Type: Artigo de periódico
Title: A Heat-stable Cytotoxic Factor Produced By Achromobacter Xylosoxidans Isolated From Brazilian Patients With Cf Is Associated With In Vitro Increased Proinflammatory Cytokines.
Author: Mantovani, Rebeca P
Levy, Carlos E
Yano, Tomomasa
Abstract: Recently, Achromobacter xylosoxidans has been related to chronic lung diseases in patients suffering from cystic fibrosis (CF), but its involvement has not been elucidated. Some virulence properties of A. xylosoxidans isolated from Brazilian patients with CF were revealed in this work. This study examined the production of a cytotoxic factor of A. xylosoxidans capable of stimulating the secretion of inflammatory cytokines (IL-6 and IL-8) from lung mucoepidermoid carcinoma cells (NCI-H292). The cytokines were measured using enzyme-linked immunosorbent (ELISA) assays. To investigate whether the cytotoxic factors may be endotoxins, they were treated with polymyxin B. The culture supernatants of all A. xylosoxidans produced a heat stable, active cytotoxin in NCI-H292 cells capable of leading to intracellular vacuoles and subsequent cell contact loss, chromatin condensation, a picnotic nucleus and cell death. There was a higher concentration of proinflammatory cytokines in the NCI-H292 cells after 24 h of incubation, with the fraction greater than 50 kDa from the culture supernatant. The cytotoxin activity remained even after treatment with polymyxin B, which suggested that the release of IL-6 and IL-8 was not stimulated by lipopolysaccharide (LPS). The cytotoxic factor produced by A. xylosoxidans may represent an important virulence factor, which when associated with CF chronic lung inflammation, may cause tissue damage and decline of lung function.
Subject: Achromobacter Denitrificans
Carcinoma, Mucoepidermoid
Cell Line, Tumor
Cell Survival
Culture Media, Conditioned
Cystic Fibrosis
Gram-negative Bacterial Infections
In Vitro Techniques
Citation: Journal Of Cystic Fibrosis : Official Journal Of The European Cystic Fibrosis Society. v. 11, n. 4, p. 305-11, 2012-Jul.
Rights: fechado
Identifier DOI: 10.1016/j.jcf.2012.02.002
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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