Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199905
Type: Artigo de periódico
Title: Survival In Vitro And Virulence Of Trypanosoma Cruzi In Açaí Pulp In Experimental Acute Chagas Disease.
Author: Barbosa, Rodrigo Labello
Dias, Viviane Liotti
Pereira, Karen Signori
Schmidt, Flávio Luis
Franco, Regina Maura Bueno
Guaraldo, Ana Maria Aparecida
Alves, Delma Pegolo
Passos, Luiz Augusto Corrêa
Abstract: Chagas disease is a parasitic infection with high socioeconomic impact throughout Latin America. Although this severe, incurable disease can be transmitted by several routes, oral transmission is currently the most important route in the Amazon Basin. Açaí pulp has nutritional properties and is popular throughout Brazil and abroad. However, this pulp has been associated with microepidemics of acute Chagas disease (ACD) in northern Brazil, where açaí fruit is the main food supplement. In this study, we examined the in vitro survival and in vivo virulence of Trypanosoma cruzi Y strain in açaí pulp. Aliquots of in natura açaí pulp produced in Belém city in the northern Brazilian state of Pará were mixed with 10⁵ trypomastigotes. The samples were incubated at room temperature or at 4 or -20°C for various periods, and the parasites were isolated by forced sieving. The resulting eluates were examined by microscopy, and the trypomastigotes were administered intraperitoneally, orally, or by gavage to immunodeficient mice (C.B-17-Prkdc(scid)/PasUnib) that had been pretreated with antibiotics. Parasitemia was quantified by the Brener method, and mortality was recorded daily. All routes of administration resulted in ACD. A 5-day delay in the onset of parasitemia occurred with oral administration. The survival and virulence of the parasites were unaffected by prior incubation at room temperature for 24 h, at 4°C for 144 h, and at -20°C for 26 h. These results indicate that T. cruzi can survive and retain its virulence in açaí pulp under various conditions and that cooling and freezing are not suitable methods for preventing foodborne ACD.
Subject: Animals
Arecaceae
Brazil
Chagas Disease
Consumer Product Safety
Food Contamination
Host-parasite Interactions
Humans
Mice
Mice, Scid
Temperature
Time Factors
Trypanosoma Cruzi
Virulence
Rights: fechado
Identifier DOI: 10.4315/0362-028X.JFP-11-233
Address: http://www.ncbi.nlm.nih.gov/pubmed/22410239
Date Issue: 2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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