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Type: Artigo de periódico
Title: Improving Metallomics Information Related To Transgenic And Non-transgenic Soybean Seeds Using 2d-hplc-icp-ms And Esi-ms/ms.
Author: Mataveli, Lidiane Raquel Verola
Fioramonte, Mariana
Gozzo, Fabio César
Arruda, Marco Aurélio Zezzi
Abstract: This work reports the use of 2D-HPLC-ICP-MS to enlarge metallomics information when considering soybean seeds. Separations using size exclusion chromatography (SEC) allowed the identification of three metal fractions: the first corresponding to molecular weights from 38.1 to 181.1 kDa, the second from 8.2 to 17.2 kDa and the third from 0.4 to 3.8 kDa. In a second dimension, using anion exchange chromatography (AEX), three sub-fractions containing Fe, Mg and Mn, one containing Cu, and three containing Co, Cu, Mg, Mn and Zn were obtained. After these separations, 33 proteins were identified using the ESI-MS/MS technique, and divided into four functional categories: plant growth/cell division, protein destination and storage, metabolism and unclassified proteins. Among the identified proteins, proteins previously related to metals were found.
Subject: Chromatography, Gel
Chromatography, High Pressure Liquid
Plant Proteins
Plants, Genetically Modified
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
Rights: aberto
Identifier DOI: 10.1039/c2mt00186a
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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