Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199812
Type: Artigo de periódico
Title: Venturi Easy Ambient Sonic-spray Ionization.
Author: Santos, Vanessa G
Regiani, Thaís
Dias, Fernanda F G
Romão, Wanderson
Jara, Jose Luis Paz
Klitzke, Clécio F
Coelho, Fernando
Eberlin, Marcos N
Abstract: The development and illustrative applications of an ambient ionization technique termed Venturi easy ambient sonic-spray ionization (V-EASI) is described. Its dual mode of operation with Venturi self-pumping makes V-EASI applicable to the direct mass spectrometric analysis of both liquid (V(L)-EASI) and solid (V(S)-EASI) samples. V-EASI is simple and easy to assemble, operating solely via the assistance of a sonic stream of nitrogen or air. The sonic gas stream causes two beneficial and integrated effects: (a) the self-pumping of solutions via the Venturi effect and (b) sonic-spray ionization (SSI) of analytes either in solution or resting on solid surfaces. In its liquid mode, V(L)-EASI is applicable to analytes in solution, forming negatively and/or positively charged intact molecular species in a soft fashion with little or no fragmentation. In its solid mode, V(S)-EASI relies on Venturi self-pumping of a proper SSI solvent solution in combination with SSI to form a stream of bipolar charged droplets that bombard the sample surface, causing desorption and ionization of the analyte molecules. As for its precursor technique (EASI), V-EASI generates bipolar droplets with considerably lower average charging, which increases selectivity for ionization with high signal-to-noise ratios and clean spectra dominated by single molecular species with minimal solvent ions. V-EASI also operates in a voltage-, heat-, and radiation-free fashion and is therefore free of thermal, electrical, or discharge interferences.
Subject: Amino Acid Sequence
Animals
Cytochromes C
Gases
Ions
Mass Spectrometry
Myoglobin
Oligopeptides
Organic Chemicals
Sound
Rights: fechado
Identifier DOI: 10.1021/ac102765z
Address: http://www.ncbi.nlm.nih.gov/pubmed/21235233
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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