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Type: Artigo de periódico
Title: Use Of Multivariate Statistical Techniques To Optimize The Simultaneous Separation Of 13 Phenolic Compounds From Extra-virgin Olive Oil By Capillary Electrophoresis.
Author: Ballus, Cristiano Augusto
Meinhart, Adriana Dillenburg
Bruns, Roy Edward
Godoy, Helena Teixeira
Abstract: Characterization of phenolic compounds in olive oil has not been achieved as yet, owing to the complexities of their chemical structures and analytical matrix. The aim of this work is to optimize and validate a method for simultaneous separation and quantification of 13 phenolic compounds from extra-virgin olive oil: tyrosol, hydroxytyrosol, oleuropein glycoside, ferrulic acid, p-coumaric acid, cinnamic acid, p-hydroxybenzoic acid, gallic acid, caffeic acid, luteolin, apigenin, vanillic acid and 3,4-dihydroxybenzoic acid. A statistical central composite design, response surface analysis and the simultaneous optimization method of Derringer and Suich were used to separate all the peaks. These multivariate procedures were efficient in determining the optimal separation condition, using five peak-pair resolutions and runtime as responses. The optimized method employed a fused-silica capillary of 50 μm i.d.× 60 cm effective length with extended light path, 50 mmol L(-1) boric acid electrolyte, 10.2 pH, 25°C, injection of 50 mbar for 25s with application of reverse voltage (-30 kV for 5s) before setting the running voltage (+30 kV) with detection at 210 nm and a run time of 12 min. Peak resolutions are found to be very sensitive to pH values outside the 10.15-10.25 range but acceptable electropherograms can be obtained for a wide range of boric acid concentrations within this pH interval.
Subject: Boric Acids
Chemical Fractionation
Electrophoresis, Capillary
Food Analysis
Hydrogen-ion Concentration
Multivariate Analysis
Plant Oils
Reproducibility Of Results
Time Factors
Citation: Talanta. v. 83, n. 4, p. 1181-7, 2011-Jan.
Rights: fechado
Identifier DOI: 10.1016/j.talanta.2010.07.013
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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