Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199800
Type: Artigo de periódico
Title: Neutrophils Of Rheumatoid Arthritis Patients On Anti-tnf-α Therapy And In Disease Remission Present Reduced Adhesive Functions In Association With Decreased Circulating Neutrophil-attractant Chemokine Levels.
Author: Dominical, V M
Bértolo, M B
Almeida, C B
Garrido, V T
Miguel, L I
Costa, F F
Conran, N
Abstract: Neutrophils participate in the initiation and progression of rheumatoid arthritis (RA) although the exact mechanisms responsible for neutrophil accumulation in rheumatoid joints are not understood. This study compared the adhesive and chemotactic functions of neutrophils from RA patients in activity (DAS28 > 3.2) and not in activity (DAS28 < 2.6) and observed the effects of different treatment approaches on these functions. Neutrophils were isolated from healthy controls (CON), and patients with active or inactive RA in use of therapy not specific for RA (NSAIDs), in use of DMARDs and in use of anti-TNF-α therapy. Adhesive and chemotactic properties were evaluated using in vitro assays; adhesion molecule expression was assessed by flow cytometry and real-time PCR and circulating chemokines were determined by ELISA. No significant alterations in the adhesive and chemotactic properties of neutrophils from active RA were observed when compared to CON neutrophils, independently of treatment regimen. In contrast, neutrophils from RA patients in disease remission presented reduced adhesive properties and a lower spontaneous chemotactic capacity, in association with decreased adhesion molecule expression, although profiles of alterations differed for those patients on DMARDs and those on anti-TNF-α therapy. Circulating levels of the major neutrophilic chemokines, IL-8 and epithelial neutrophil activating peptide-78, were also significantly decreased in those patients demonstrating a clinical response. Remission of RA appears to be associated with ameliorations in aspects important for neutrophil adhesion and chemotaxis; whether these alterations contribute to decrease neutrophil migration to the synovial fluid, with consequent improvements in the clinical manifestations of RA, remains to be determined.
Subject: Adult
Aged
Antibodies, Monoclonal
Antirheumatic Agents
Arthritis, Rheumatoid
Case-control Studies
Cell Adhesion
Cell Adhesion Molecules
Chemokines
Chemotaxis, Leukocyte
Female
Gene Expression
Humans
Interleukin-8
L-selectin
Male
Middle Aged
Neutrophils
Remission Induction
Tumor Necrosis Factor-alpha
Young Adult
Rights: fechado
Identifier DOI: 10.1111/j.1365-3083.2011.02503.x
Address: http://www.ncbi.nlm.nih.gov/pubmed/21208248
Date Issue: 2011
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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