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Type: Artigo de periódico
Title: Behavior Of Wild-type And Transfected S2 Cells Cultured In Two Different Media.
Author: Batista, Fabiana R X
Greco, Kátia N
Astray, Renato M
Jorge, Soraia A C
Augusto, Elisabeth F P
Pereira, Carlos A
Mendonça, Ronaldo Z
Moraes, Angela M
Abstract: An animal protein-free medium composed of IPL-41 containing 6 g L(-1) yeastolate ultrafiltrate, 10 g L(-1) glucose, 2 g L(-1) lactose, 5 g L(-1) glutamine, 1% lipid emulsion, and 0.1% Pluronic F-68 was used for producing recombinant proteins in batch mode employing two cell lines, S2AcRVGP2k expressing the G glycoprotein from rabies virus (RVGP) and S2AcHBsAgHy-9C expressing the surface antigen of hepatitis B virus (HBsAg), both obtained from Drosophila melanogaster S2 cells. Growth of wild-type S2 cells was also evaluated in the same medium. Cell behavior in the tested medium was compared to that verified in Sf900 II®. The results show that in shake flasks, S2AcRVGP2k and S2AcHBsAgHy-9C cells reached around 2 × 10(7) cells mL(-1) in both media. In supplemented IPL-41 and Sf900 II® media, S2AcRVGP2k cells produced 367 ng RVGP mL(-1) and 638 ng RVGP mL(-1), respectively, while S2AcHBsAgHy-9C cells correspondently produced 573 ng HBsAg mL(-1) and 322 ng HBsAg mL(-1) in the mentioned media. In stirred tanks, S2AcRVGP2k cells reached 3 × 10(7) cells mL(-1) and produced up to 758 ng RVGP mL(-1). In general, glucose was consumed by cells, while lactate and ammonia were produced.
Subject: Animals
Cell Culture Techniques
Cell Line
Culture Media, Serum-free
Drosophila Melanogaster
Gene Expression
Hepatitis B Virus
Rabies Virus
Recombinant Proteins
Viral Proteins
Rights: fechado
Identifier DOI: 10.1007/s12010-010-8918-z
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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