Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199785
Type: Artigo de periódico
Title: Surface Miscibility Of Epc/dotap/dope In Binary And Ternary Mixed Monolayers.
Author: Rigoletto, Thais de Paula
Zaniquelli, Maria Elisabete Darbello
Santana, Maria Helena Andrade
de la Torre, Lucimara Gaziola
Abstract: Surface pressure (π)-molecular area (A) curves were used to characterize the packing of pseudo-ternary mixed Langmuir monolayers of egg phosphatidylcholine (EPC), 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) and L-α-dioleoyl phosphatidylethanolamine (DOPE). This pseudo-ternary mixture EPC/DOPE/DOTAP has been successfully employed in liposome formulations designed for DNA non-viral vectors. Pseudo-binary mixtures were also studied as a control. Miscibility behavior was inferred from π-A curves applying the additivity rule by calculating the excess free energy of mixture (ΔG(Exc)). The interaction between the lipids was also deduced from the surface compressional modulus (C(s)(-1)). The deviation from ideality shows dependence on the lipid polar head type and monolayer composition. For lower DOPE concentrations, the forces are predominantly attractive. However, if the monolayer is DOPE rich, the DOTAP presence disturbs the PE-PE intermolecular interaction and the net interaction is then repulsive. The ternary monolayer EPC/DOPE/DOTAP presented itself in two configurations, modulated by the DOPE content, in a similar behavior to the DOPE/DOTAP monolayers. These results contribute to the understanding of the lipid interactions and packing in self-assembled systems associated with the in vitro and in vivo stability of liposomes.
Subject: Egg Yolk
Fatty Acids, Monounsaturated
Lipid Bilayers
Membranes, Artificial
Phosphatidylcholines
Phosphatidylethanolamines
Quaternary Ammonium Compounds
Surface Properties
Thermodynamics
Rights: fechado
Identifier DOI: 10.1016/j.colsurfb.2010.11.030
Address: http://www.ncbi.nlm.nih.gov/pubmed/21159494
Date Issue: 2011
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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