Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199781
Type: Artigo de periódico
Title: Ferric Iron Uptake Genes Are Differentially Expressed In The Presence Of Copper Sulfides In Acidithiobacillus Ferrooxidans Strain Lr.
Author: Ferraz, Lúcio F C
Verde, Leandro C L
Vicentini, Renato
Felício, Ana P
Ribeiro, Marcelo L
Alexandrino, Fabiana
Novo, Maria T M
Garcia, Oswaldo
Rigden, Daniel J
Ottoboni, Laura M M
Abstract: Acidithiobacillus ferrooxidans is one of the most widely used microorganisms in bioleaching operations to recover copper from low-grade copper sulfide ores. This work aimed to investigate the relative expression of genes related to the iron uptake system when A. ferrooxidans LR was maintained in contact with chalcopyrite or bornite as the sole energy source. Real-time quantitative PCR analysis revealed that the presence of bornite had no effect on the expression of seven genes related to the siderophore-mediated Fe(III) uptake system, while in the presence of chalcopyrite the expression of the genes was up-regulated. Bioinformatic analysis of the genomic region where these genes were found revealed the existence of three new putative DNA-binding sequences for the ferric iron uptake transcriptional regulator (Fur). Electrophoretic mobility shift assays demonstrated that a purified A. ferrooxidans His-tagged Fur protein was able to bind in vitro to each of these putative Fur boxes, suggesting that Fur regulated the expression of these genes. The expression of fur and two known Fur-regulated genes, mntH and dsrK, was also investigated in the presence of chalcopyrite. While the expression of fur and mntH was up-regulated, the expression of dsrK was down-regulated. The low amount of ferrous iron in the medium was probably responsible for the up-regulation of fur and the genes related to the siderophore-mediated Fe(III) uptake system when A. ferrooxidans LR was kept in the presence of chalcopyrite. A homology model of the A. ferrooxidans Fur was constructed and revealed that the putative DNA-binding surface presents conserved positively charged residues, supporting a previously suggested mode of interaction with DNA. The up-regulation of fur and the siderophore-mediated Fe(III) uptake genes, and the down-regulation of dsrK suggest that in the presence of chalcopyrite Fur acts as a transcription inducer and repressor.
Subject: Acidithiobacillus
Bacterial Proteins
Computational Biology
Copper
Electrophoretic Mobility Shift Assay
Gene Expression Regulation, Bacterial
Iron
Polymerase Chain Reaction
Sulfides
Rights: fechado
Identifier DOI: 10.1007/s10482-010-9533-2
Address: http://www.ncbi.nlm.nih.gov/pubmed/21132364
Date Issue: 2011
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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