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Type: Artigo de periódico
Title: Simultaneous Extraction And Analysis By High Performance Liquid Chromatography Coupled To Diode Array And Mass Spectrometric Detectors Of Bixin And Phenolic Compounds From Annatto Seeds.
Author: Chisté, Renan Campos
Yamashita, Fábio
Gozzo, Fábio Cesar
Mercadante, Adriana Zerlotti
Abstract: This study was designed to identify and quantify the carotenoids and phenolic compounds from annatto seeds using high performance liquid chromatography coupled to diode array and mass spectrometer detectors (HPLC-DAD-MS/MS). Furthermore, using response surface methodology, an optimized procedure for simultaneous extraction of these compounds was established. In addition to bixin, known to be the main carotenoid in annatto seeds, hypolaetin and a caffeoyl acid derivative were identified as the main phenolic compounds. The optimized procedure involved 15 extractions using acetone:methanol:water (50:40:10, v/v/v) as solvent, a solid-liquid ratio of 1:9 (m/v) and an extraction time of 5 min. Validation data indicated that the HPLC method proposed provided good linearity, sensitivity, procedure accuracy, system precision and suggested its suitability for the simultaneous analysis of phenolic compounds and carotenoids in annatto seeds.
Subject: Bixaceae
Chromatography, High Pressure Liquid
Linear Models
Models, Chemical
Reproducibility Of Results
Tandem Mass Spectrometry
Citation: Journal Of Chromatography. A. v. 1218, n. 1, p. 57-63, 2011-Jan.
Rights: fechado
Identifier DOI: 10.1016/j.chroma.2010.10.094
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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