Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199708
Type: Artigo de periódico
Title: The Small Heat Shock Proteins From Acidithiobacillus Ferrooxidans: Gene Expression, Phylogenetic Analysis, And Structural Modeling.
Author: Ribeiro, Daniela A
Bem, Luiz E V Del
Vicentini, Renato
Ferraz, Lúcio F C
Murakami, Mario T
Ottoboni, Laura M M
Abstract: Acidithiobacillus ferrooxidans is an acidophilic, chemolithoautotrophic bacterium that has been successfully used in metal bioleaching. In this study, an analysis of the A. ferrooxidans ATCC 23270 genome revealed the presence of three sHSP genes, Afe_1009, Afe_1437 and Afe_2172, that encode proteins from the HSP20 family, a class of intracellular multimers that is especially important in extremophile microorganisms. The expression of the sHSP genes was investigated in A. ferrooxidans cells submitted to a heat shock at 40°C for 15, 30 and 60 minutes. After 60 minutes, the gene on locus Afe_1437 was about 20-fold more highly expressed than the gene on locus Afe_2172. Bioinformatic and phylogenetic analyses showed that the sHSPs from A. ferrooxidans are possible non-paralogous proteins, and are regulated by the σ32 factor, a common transcription factor of heat shock proteins. Structural studies using homology molecular modeling indicated that the proteins encoded by Afe_1009 and Afe_1437 have a conserved α-crystallin domain and share similar structural features with the sHSP from Methanococcus jannaschii, suggesting that their biological assembly involves 24 molecules and resembles a hollow spherical shell. We conclude that the sHSPs encoded by the Afe_1437 and Afe_1009 genes are more likely to act as molecular chaperones in the A. ferrooxidans heat shock response. In addition, the three sHSPs from A. ferrooxidans are not recent paralogs, and the Afe_1437 and Afe_1009 genes could be inherited horizontally by A. ferrooxidans.
Subject: Acidithiobacillus
Amino Acid Sequence
Bacterial Proteins
Base Sequence
Computational Biology
Gene Expression Regulation, Bacterial
Heat-shock Proteins, Small
Hot Temperature
Models, Molecular
Molecular Sequence Data
Phylogeny
Protein Structure, Tertiary
Sequence Analysis, Protein
Rights: fechado
Identifier DOI: 10.1186/1471-2180-11-259
Address: http://www.ncbi.nlm.nih.gov/pubmed/22151959
Date Issue: 2011
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

Files in This Item:
File SizeFormat 
pmed_22151959.pdf1.68 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.