Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199694
Type: Artigo de periódico
Title: Effects Of Multi-walled Carbon Nanotubes (mwcnt) Under Neisseria Meningitidis Transformation Process.
Author: Mattos, Ives B
Alves, Danilo A
Hollanda, Luciana M
Ceragiogli, Helder J
Baranauskas, Vitor
Lancellotti, Marcelo
Abstract: This study aimed at verifying the action of multi-walled carbon nanotubes (MWCNT) under the naturally transformable Neisseria meningitidis against two different DNA obtained from isogenic mutants of this microorganism, an important pathogen implicated in the genetic horizontal transfer of DNA, causing the escape of the principal vaccination measured worldwide by the capsular switching process. The bacterium receptor strain C2135 was cultivated and had its mutant DNA donor M2 and M6, which received a receptor strain and MWCNT at three different concentrations. The inhibition effect of DNAse on the DNA in contact with nanoparticles was evaluated. The results indicated an in increase in the transformation capacity of N. meninigtidis in different concentrations of MWCNT when compared with negative control without nanotubes. A final analysis of the interaction between DNA and MWCNT was carried out using Raman Spectroscopy. These increases in the transformation capacity mediated by MWCNT, in meningococci, indicate the interaction of these particles with the virulence acquisition of these bacteria, as well as with the increase in the vaccination escape process.
Subject: Bacterial Capsules
Gene Transfer Techniques
Gene Transfer, Horizontal
Humans
Nanotubes, Carbon
Neisseria Meningitidis
Spectrum Analysis, Raman
Transformation, Bacterial
Rights: aberto
Identifier DOI: 10.1186/1477-3155-9-53
Address: http://www.ncbi.nlm.nih.gov/pubmed/22088149
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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