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Type: Artigo de periódico
Title: A Role For Ferritin In The Antioxidant System In Coffee Cell Cultures.
Author: Bottcher, Alexandra
Nobile, Paula Macedo
Martins, Paula Fabiane
Conte, Fábio Frangiotti
Azevedo, Ricardo Antunes
Mazzafera, Paulo
Abstract: Iron (Fe) is an essential nutrient for plants, but it can generate oxidative stress at high concentrations. In this study, Coffea arabica L. cell suspension cultures were exposed to excess Fe (60 and 240 μM) to investigate changes in the gene expression of ferritin and antioxidant enzymes. Iron content accumulated during cell growth, and Western blot analysis showed an increase of ferritin in cells treated with Fe. The expression of two ferritin genes retrieved from the Brazilian coffee EST database was studied. CaFER1, but not CaFER2, transcripts were induced by Fe exposure. Phylogenetic analysis revealed that CaFER1 is not similar to CaFER2 or to any ferritin that has been characterised in detail. The increase in ferritin gene expression was accompanied by an increase in the activity of antioxidant enzymes. Superoxide dismutase, guaiacol peroxidase, catalase, and glutathione reductase activities increased in cells grown in the presence of excess Fe, especially at 60 μM, while the activity of glutathione S-transferase decreased. These data suggest that Fe induces oxidative stress in coffee cell suspension cultures and that ferritin participates in the antioxidant system to protect cells against oxidative damage. Thus, cellular Fe concentrations must be finely regulated to avoid cellular damage most likely caused by increased oxidative stress induced by Fe. However, transcriptional analyses indicate that ferritin genes are differentially controlled, as only CaFER1 expression was responsive to Fe treatment.
Subject: Amino Acid Sequence
Base Sequence
Blotting, Western
Expressed Sequence Tags
Glutathione Reductase
Molecular Sequence Data
Oxidative Stress
Plant Proteins
Polymerase Chain Reaction
Sequence Alignment
Superoxide Dismutase
Rights: fechado
Identifier DOI: 10.1007/s10534-010-9388-z
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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