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dc.typeArtigo de periódicopt
dc.titleTlr2 And Tlr4 Gene Promoter Methylation Status During Chronic
dc.contributor.authorDe Oliveira, Naila Francis Paulopt
dc.contributor.authorAndia, Denise Carletopt
dc.contributor.authorPlanello, Aline Cristianept
dc.contributor.authorPasetto, Silvanapt
dc.contributor.authorMarques, Marcelo Rochapt
dc.contributor.authorNociti, Francisco Humbertopt
dc.contributor.authorLine, Sérgio Roberto Perespt
dc.contributor.authorDe Souza, Ana Paulapt
unicamp.authorNaila Francis Paulo De Oliveira, Department of Morphology, Laboratory of Molecular Biology, Division of Histology, Piracicaba Dental School, University of Campinas-UNICAMP, Piracicaba, São Paulo, SP, Carleto Andia,pt Cristiane Planello,pt Pasetto,pt Rocha Marques,pt Humberto Nociti,ptérgio Roberto Peres Line,pt Paula De Souza,pt
dc.subjectCase-control Studiespt
dc.subjectChi-square Distributionpt
dc.subjectChronic Periodontitispt
dc.subjectCpg Islandspt
dc.subjectDna Methylationpt
dc.subjectMiddle Agedpt
dc.subjectPromoter Regions, Geneticpt
dc.subjectReverse Transcriptase Polymerase Chain Reactionpt
dc.subjectStatistics, Nonparametricpt
dc.subjectToll-like Receptor 2pt
dc.subjectToll-like Receptor 4pt
dc.description.abstractThe objective of this study was to analyse the status of DNA methylation in the promoter region of the toll-like receptor (TLR)2 and TLR4 genes in gingival tissue samples from healthy subjects, smokers and non-smokers affected by chronic periodontitis. Genomic DNA and total RNA were purified from gingival tissue using the TRIZOL reagent protocol. Genomic DNA was then digested by methylation-sensitive restriction enzymes, amplified by polymerase chain reaction (PCR), electrophoresed on a 10% polyacrylamide gel and stained using SYBR Gold. Real-time PCR was also performed to verify the transcript levels. The CpG dinucleotides analysed were observed to be unmethylated in the majority of DNA samples of the three groups and statistical differences were not found among groups (p>0.05). However, a trend towards methylation was observed in the TLR2 HhaI site in the samples of the periodontitis non-smoker groups. In fact, the analysis of all CpG sites together shows which complete methylation is observed in the shortest level in the samples of periodontitis non-smoker group. The analysis of transcript levels demonstrated no difference among groups (p>0.05). The results demonstrated major unmethylation of the TLR4 gene promoter in all groups. However, the results for the TLR2 gene promoter are inconclusive; this gene was found as a mosaic of methylated and unmethylated DNA in the majority of samples of the three groups and we also observed a trend towards the DNA methylation of CpG sites recognized by the HhaI enzyme.en
dc.relation.ispartofJournal Of Clinical Periodontologyen
dc.relation.ispartofabbreviationJ. Clin.
dc.identifier.citationJournal Of Clinical Periodontology. v. 38, n. 11, p. 975-83,
dc.rights.holder© 2011 John Wiley & Sons A/
dc.description.provenanceMade available in DSpace on 2015-11-27T13:21:52Z (GMT). No. of bitstreams: 1 pmed_21899586.pdf: 209664 bytes, checksum: d5afd4831b3b95bc71bd854f0d601e86 (MD5) Previous issue date: 2011en
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