Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199617
Type: Artigo de periódico
Title: N-acetylcysteine Protects Pancreatic Islet Against Glucocorticoid Toxicity.
Author: Roma, Letícia P
Oliveira, Camila A M
Carneiro, Everardo M
Albuquerque, Gabriela G
Boschero, Antonio C
Souza, Kleber L A
Abstract: Reactive oxygen species (ROS) are involved in many physiological and pathological processes. In the present study, we analysed whether the synthetic glucocorticoid dexamethasone induces oxidative stress in cultured pancreatic islets and whether the effects of dexamethasone on insulin secretion, gene expression, and viability can be counteracted by concomitant incubation with N-acetylcysteine (NAC). ROS production was measured by dichlorofluorescein (DCFH-DA) assay, insulin secretion by radioimmunoassay, intracellular calcium dynamics by fura-2-based fluorescence, gene expression by real-time polymerase chain reaction analyses and cell viability by the MTS assay. Dexamethasone (Dexa) increased ROS production and decreased glucose-stimulated insulin secretion after 72 hours incubation. Intracellular ROS levels were decreased and the insulin secretion capacity was recovered by concomitant treatment with Dexa+NAC. The total insulin content and intracellular Ca2+ levels were not modulated in either Dexa or Dexa+NAC groups. There was a decrease in the NAD(P)H production, used as an indicator of viability, after dexamethasone treatment. Concomitant incubation with NAC returned viability to control levels. Dexa also decreased synaptotagmin VII (SYT VII) gene expression. In contrast, the Dexa+NAC group demonstrated an increased expression of SYT VII compared to controls. Surprisingly, treatment with NAC decreased the gene expression of the antioxidant enzyme copper zinc superoxide dismutase soluble. Our results indicate that dexamethasone increases ROS production, decreases viability, and impairs insulin secretion in pancreatic rat islets. These effects can be counteracted by NAC, which not only decreases ROS levels but also modulates the expression of genes involved in the secretory pathway and those coding for antioxidant enzymes.
Subject: Acetylcysteine
Animals
Cell Survival
Cells, Cultured
Dexamethasone
Free Radical Scavengers
Free Radicals
Glucocorticoids
Insulin
Islets Of Langerhans
Oxidative Stress
Protective Agents
Rats
Rats, Sprague-dawley
Rats, Wistar
Reactive Oxygen Species
Synaptotagmins
Rights: fechado
Identifier DOI: 10.1179/1351000211Y.0000000006
Address: http://www.ncbi.nlm.nih.gov/pubmed/21888768
Date Issue: 2011
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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