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Type: Artigo de periódico
Title: Genotypic And Phenotypic Analysis Of S. Mutans Isolated From Dental Biofilms Formed In Vivo Under High Cariogenic Conditions.
Author: Arthur, Rodrigo Alex
Cury, Altair Antoninha Del Bel
Graner, Renata Oliveira Mattos
Rosalen, Pedro Luiz
Vale, Gláuber Campos
Paes Leme, Adriana Franco
Cury, Jaime Aparecido
Tabchoury, Cínthia Pereira Machado
Abstract: The oral cavity harbors several Streptococcus mutans genotypes, which could present distinct virulence properties. However, little is known about the diversity and virulence traits of S. mutans genotypes isolated in vivo under controlled conditions of high cariogenic challenge. This study evaluated the genotypic diversity of S. mutans isolated from dental biofilms formed in vivo under sucrose exposure, as well as their acidogenicity and aciduricity. To form biofilms, subjects rinsed their mouths with distilled water or sucrose solution 8 times/day for 3 days. S. mutans collected from saliva and biofilms were genotyped by arbitrarily-primed PCR. Genotypes identified in the biofilms were evaluated regarding their ability to lower the suspension pH through glycolysis and their acid susceptibility and F-ATPase activity. Most subjects harbored only one genotype in saliva, which was detected in almost all biofilm samples at high proportions. Genotypes isolated only in the presence of sucrose had higher acidogenicity than those isolated only in the presence of water. Genotypes from biofilms formed with sucrose were more aciduric after 30 and 60 min of incubation at pH 2.8 and 5.0, respectively. The present results suggest that biofilms formed under high cariogenic conditions may harbor more aciduric and acidogenic S. mutans genotypes.
Subject: Acids
Bacterial Proton-translocating Atpases
Cariogenic Agents
Cross-over Studies
Dental Deposits
Genetic Variation
Hydrogen-ion Concentration
Microbial Viability
Polymerase Chain Reaction
Streptococcus Mutans
Time Factors
Young Adult
Rights: fechado
Identifier DOI: 
Date Issue: 2011
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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