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Type: Artigo de periódico
Title: Improvement Of Phytase Activity By A New Saccharomyces Cerevisiae Strain Using Statistical Optimization.
Author: Ries, Edi Franciele
Alves Macedo, Gabriela
Abstract: Using statistical optimization, we enhanced the activity of phytase by a new Saccharomyces cerevisiae strain cultured in mineral medium. Concentrations of carbon source and inducer of phytase production were optimized using a 2(2) full factorial CCD and response surface methodology (RSM). Urea was fixed as nitrogen source in culture medium (0.15%, w/v). The culture medium consisting of 2.5% sucrose and 0.5% sodium phytate optimally supported the maximum phytase activity. In addition, we found that culture of the yeast at 35°C with shaking at 150 rpm supports maximum phytase production. The validity of this model was verified by culturing the organisms in flasks on a shaker. Using the optimized media and growth conditions, we obtained a 10-fold improvement in the production of phytase by S. cerevisiae.
Citation: Enzyme Research. v. 2011, p. 796394, 2011.
Rights: aberto
Identifier DOI: 10.4061/2011/796394
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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