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Type: Artigo de periódico
Title: Lc-ms/ms Quantitation Of Plasma Progesterone In Cattle.
Author: Fernandes, R M T
Gomes, G C
Porcari, A M
Pimentel, J R V
Porciúncula, P M
Martins-Júnior, H A
Miguez, P H P
da Costa, J L
Amaral, P H
Perecin, F
Meurer, E C
Furtado, P V
Simas, R C
Eberlin, M N
Ferreira, C R
Madureira, E H
Abstract: Quantitation of progesterone (P(4)) in biological fluids is often performed by radioimmunoassay (RIA), whereas liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) has been used much less often. Due to its autoconfirmatory nature, LC-MS/MS greatly minimizes false positives and interference. Herein we report and compare with RIA an optimized LC-MS/MS method for rapid, efficient, and cost-effective quantitation of P(4) in plasma of cattle with no sample derivatization. The quantitation of plasma P(4) released from three nonbiodegradable, commercial, intravaginal P(4)-releasing devices (IPRD) over 192 h in six ovariectomized cows was compared in a pairwise study as a test case. Both techniques showed similar P(4) kinetics (P > 0.05) whereas results of P(4) quantitation by RIA were consistently higher compared with LC-MS/MS (P < 0.05) due to interference and matrix effects. The LC-MS/MS method was validated according to the recommended analytical standards and displayed P(4) limits of detection (LOD) and quantitation (LOQ) of 0.08 and a 0.25 ng/mL, respectively. The high selective LC-MS/MS method proposed herein for P(4) quantitation eliminates the risks associated with radioactive handling; it also requires no sample derivatization, which is a common requirement for LC-MS/MS quantitation of steroid hormones. Its application to multisteroid assays is also viable, and it is envisaged that it may provide a gold standard technique for hormone quantitation in animal reproductive science studies.
Subject: Animals
Chromatography, Liquid
Estrus Synchronization
Tandem Mass Spectrometry
Citation: Theriogenology. v. 76, n. 7, p. 1266-74.e1-2, 2011-Oct.
Rights: fechado
Identifier DOI: 10.1016/j.theriogenology.2011.05.033
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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