Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199520
Type: Artigo de periódico
Title: Molecular Characterization Of A Miraculin-like Gene Differentially Expressed During Coffee Development And Coffee Leaf Miner Infestation.
Author: Mondego, Jorge Maurício Costa
Duarte, Melina Pasini
Kiyota, Eduardo
Martínez, Leandro
de Camargo, Sandra Rodrigues
De Caroli, Fernanda P
Alves, Beatriz Santos Capela
Guerreiro, Sandra Maria Carmello
Oliva, Maria Luiza Vilela
Guerreiro-Filho, Oliveiro
Menossi, Marcelo
Abstract: The characterization of a coffee gene encoding a protein similar to miraculin-like proteins, which are members of the plant Kunitz serine trypsin inhibitor (STI) family of proteinase inhibitors (PIs), is described. PIs are important proteins in plant defence against insects and in the regulation of proteolysis during plant development. This gene has high identity with the Richadella dulcifica taste-modifying protein miraculin and with the tomato protein LeMir; and was named as CoMir (Coffea miraculin). Structural protein modelling indicated that CoMir had structural similarities with the Kunitz STI proteins, but suggested specific folding structures. CoMir was up-regulated after coffee leaf miner (Leucoptera coffella) oviposition in resistant plants of a progeny derived from crosses between C. racemosa (resistant) and C. arabica (susceptible). Interestingly, this gene was down-regulated during coffee leaf miner herbivory in susceptible plants. CoMir expression was up-regulated after abscisic acid application and wounding stress and was prominent during the early stages of flower and fruit development. In situ hybridization revealed that CoMir transcripts accumulated in the anther tissues that display programmed cell death (tapetum, endothecium and stomium) and in the metaxylem vessels of the petals, stigma and leaves. In addition, the recombinant protein CoMir shows inhibitory activity against trypsin. According to the present results CoMir may act in proteolytic regulation during coffee development and in the defence against L. coffeella. The similarity of CoMir with other Kunitz STI proteins and the role of CoMir in plant development and plant stress are discussed.
Subject: Amino Acid Sequence
Animals
Base Sequence
Blotting, Southern
Coffee
Gene Expression Profiling
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Genes, Plant
Glycoproteins
Models, Molecular
Molecular Sequence Data
Moths
Organ Specificity
Phylogeny
Plant Proteins
Protein Processing, Post-translational
Rna, Messenger
Sequence Alignment
Sequence Analysis, Dna
Rights: fechado
Identifier DOI: 10.1007/s00425-010-1284-9
Address: http://www.ncbi.nlm.nih.gov/pubmed/20931223
Date Issue: 2011
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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