Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199495
Type: Artigo de periódico
Title: Molecular Phylogenetic Relationships And Phenotypic Diversity In Miniaturized Toadlets, Genus Brachycephalus (amphibia: Anura: Brachycephalidae).
Author: Clemente-Carvalho, Rute B G
Klaczko, Julia
Ivan Perez, S
Alves, Ana C R
Haddad, Célio F B
dos Reis, Sérgio F
Abstract: Toadlets of the genus Brachycephalus are endemic to the Atlantic rainforests of southeastern and southern Brazil. The 14 species currently described have snout-vent lengths less than 18 mm and are thought to have evolved through miniaturization: an evolutionary process leading to an extremely small adult body size. Here, we present the first comprehensive phylogenetic analysis for Brachycephalus, using a multilocus approach based on two nuclear (Rag-1 and Tyr) and three mitochondrial (Cyt b, 12S, and 16S rRNA) gene regions. Phylogenetic relationships were inferred using a partitioned Bayesian analysis of concatenated sequences and the hierarchical Bayesian method (BEST) that estimates species trees based on the multispecies coalescent model. Individual gene trees showed conflict and also varied in resolution. With the exception of the mitochondrial gene tree, no gene tree was completely resolved. The concatenated gene tree was completely resolved and is identical in topology and degree of statistical support to the individual mtDNA gene tree. On the other hand, the BEST species tree showed reduced significant node support relative to the concatenate tree and recovered a basal trichotomy, although some bipartitions were significantly supported at the tips of the species tree. Comparison of the log likelihoods for the concatenated and BEST trees suggests that the method implemented in BEST explains the multilocus data for Brachycephalus better than the Bayesian analysis of concatenated data. Landmark-based geometric morphometrics revealed marked variation in cranial shape between the species of Brachycephalus. In addition, a statistically significant association was demonstrated between variation in cranial shape and genetic distances estimated from the mtDNA and nuclear loci. Notably, B. ephippium and B. garbeana that are predicted to be sister-species in the individual and concatenated gene trees and the BEST species tree share an evolutionary novelty, the hyperossified dorsal plate.
Subject: Animals
Anura
Base Sequence
Bayes Theorem
Biological Evolution
Brazil
Dna, Mitochondrial
Dna, Ribosomal
Evolution, Molecular
Mitochondria
Molecular Sequence Data
Multilocus Sequence Typing
Phenotype
Phylogeny
Rna, Ribosomal, 16s
Sequence Analysis, Dna
Skull
Species Specificity
Rights: fechado
Identifier DOI: 10.1016/j.ympev.2011.05.017
Address: http://www.ncbi.nlm.nih.gov/pubmed/21693192
Date Issue: 2011
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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