Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199442
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleEffects Of Plasmodium Berghei On Thymus: High Levels Of Apoptosis And Premature Egress Of Cd4(+)cd8(+) Thymocytes In Experimentally Infected Mice.pt_BR
dc.contributor.authorFrancelin, Carolinapt_BR
dc.contributor.authorPaulino, Luciana Campospt_BR
dc.contributor.authorGameiro, Jacypt_BR
dc.contributor.authorVerinaud, Lianapt_BR
unicamp.authorCarolina Francelin, Department of Anatomy, Cell Biology and Physiology, Biology Institute, State University of Campinas - UNICAMP, Barão Geraldo, Campinas CEP: 13083-970, São Paulo, Brazil.pt_BR
unicamp.author.externalLuciana Campos Paulino,pt
unicamp.author.externalJacy Gameiro,pt
unicamp.author.externalLiana Verinaud,pt
dc.subjectAnimalspt_BR
dc.subjectAntigens, Cd4pt_BR
dc.subjectAntigens, Cd8pt_BR
dc.subjectApoptosispt_BR
dc.subjectImmunophenotypingpt_BR
dc.subjectLymph Nodespt_BR
dc.subjectMalariapt_BR
dc.subjectMalept_BR
dc.subjectMicept_BR
dc.subjectMice, Inbred Balb Cpt_BR
dc.subjectPlasmodium Bergheipt_BR
dc.subjectThymocytespt_BR
dc.subjectThymus Glandpt_BR
dc.description.abstractWe have previously showed alterations in the thymus during experimental infection with Plasmodium berghei, the causative agent of Malaria. Such alterations comprised histological changes with loss of delimitation between cortical and medullar regions, a profound atrophy with depletion of CD4(+)CD8(+) double-positive (DP) thymocytes, and severe changes in the expression of cell migration-related molecules, belonging to the extracellular matrix and chemokine protein families. Taken together, these considerations prompted us to evaluate if the acute thymic atrophy observed during Plasmodium infection was correlated with increased apoptotic levels of thymocytes or with their premature emigration to the periphery. Our results confirmed that the marked reduction of the thymus weight in infected animals was accompanied by histological alterations, which included a very large number of cells showing nuclear condensation and karyorrhectic changes surrounded by histiocytes suggesting increased levels of apoptosis. This was confirmed by immunohistochemistry and flow cytometry techniques. In order to verify if an accelerated emigration of thymic cells to the peripheral lymphoid organs was also occurring we analyzed the spleen and mesenteric lymph nodes from control and infected mice. No significant differences were found in the spleen, but were seen after 14 days of infection between control and infected mice in the mesenteric lymph nodes. The main alteration was the presence of double negative (CD4(-)CD8(-)) and double positive (CD4(+)CD8(+)) cells. We concluded that both apoptosis of thymocytes and premature egress of immature cells take place during infection. Additional studies will be necessary to verify how such alterations might influence the systemic immune response to the parasite.en
dc.relation.ispartofImmunobiologypt_BR
dc.relation.ispartofabbreviationImmunobiologypt_BR
dc.date.issued2011-Octpt_BR
dc.identifier.citationImmunobiology. v. 216, n. 10, p. 1148-54, 2011-Oct.pt_BR
dc.language.isoengpt_BR
dc.description.volume216pt_BR
dc.description.firstpage1148-54pt_BR
dc.rightsfechadopt_BR
dc.rights.holderCopyright © 2011 Elsevier GmbH. All rights reserved.pt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1878-3279pt_BR
dc.identifier.doi10.1016/j.imbio.2011.03.009pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/21601941pt_BR
dc.date.available2015-11-27T13:21:27Z-
dc.date.accessioned2015-11-27T13:21:27Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:21:27Z (GMT). No. of bitstreams: 1 pmed_21601941.pdf: 1078546 bytes, checksum: 96dbeba44e5d2e9d37f2a67266fc5228 (MD5) Previous issue date: 2011en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199442-
dc.identifier.idPubmed21601941pt_BR
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