Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199440
Type: Artigo de periódico
Title: Indigofera Suffruticosa Mill As New Source Of Healing Agent: Involvement Of Prostaglandin And Mucus And Heat Shock Proteins.
Author: Luiz-Ferreira, Anderson
Cola, Maira
Barbastefano, Victor
Farias-Silva, Elisangela
Calvo, Tamara Regina
de Almeida, Ana Beatriz Albino
Pellizzon, Claudia Helena
Hiruma-Lima, Clélia Akiko
Vilegas, Wagner
Souza-Brito, Alba Regina Monteiro
Abstract: Indigofera suffruticosa is specie typical of the Cerrado or Brazilian savannah; it is a member of the Fabaceae family - in folkmedicine is used for gastric disorders, infection and inflammation. Ethyl acetate fraction (AcF) and aqueous fraction (AqF) of the methanolic extract of I. suffruticosa leaves were evaluated against acute gastric ulcer. The AcF fraction was selected to assess its activity in ulcer healing and its gastroprotective effects via mucus and gastric secretion. The gastroprotective action of AcF and AqF fractions were evaluated in a rodent experimental model. The action mechanisms, involvements of the antisecretory action, mucus and prostaglandin production, toxicological and healing activity of the AcF (100mg/kg, p.o.) were evaluated. We also used histological analysis (HE and PAS) and immunohistochemical (PCNA and HSP-70) assays to evaluate the effects of I. suffruticosa. AcF significantly inhibited the gastric mucosal damage caused by ethanol. This effect was statistically significant in 100mg/kg group compared vehicle. AcF did not interfered with gastric secretion, significantly increased the PGE(2) and mucus production (validated in PAS technique). The gastroprotection was attenuated by pretreatment with N-ethylmaleimide, but not L-NAME. In acid-acetic-induced ulcer model AcF accelerated ulcer healing. Immunohistochemistry analysis showed induction of proliferating cell (PCNA) and heat shock protein (HSP 70). These results showed that AcF acted as gastroprotective agent stimulating prostaglandin, mucus and HSP70.
Subject: Acetates
Animals
Anti-ulcer Agents
Cytoprotection
Disease Models, Animal
Ethanol
Hsp70 Heat-shock Proteins
Immunohistochemistry
Indigofera
Male
Methanol
Mucus
Nitric Oxide
Plant Extracts
Proliferating Cell Nuclear Antigen
Prostaglandins
Rats
Solvents
Stomach
Stomach Ulcer
Sulfhydryl Compounds
Water
Wound Healing
Rights: fechado
Identifier DOI: 10.1016/j.jep.2011.05.006
Address: http://www.ncbi.nlm.nih.gov/pubmed/21600972
Date Issue: 2011
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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