Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199437
Type: Artigo de periódico
Title: Ex Vivo Expansion Of Cd56+ Nk And Nkt-like Lymphocytes From Peripheral Blood Mononuclear Cells Of Patients With Ovarian Neoplasia.
Author: Alves, P C M
Andrade, L A L De Angelo
Petta, C A
Lorand-Metze, I
Derchain, S F
Guimarães, F
Abstract: Methods for ex vivo expansion of natural killer (NK) cells have allowed obtaining enough numbers of human NK cells for clinical trials. However, the evaluation of these methods has been mostly limited to haematological malignancies. This study aimed at evaluating a method for selective expansion of NK cells when applied in peripheral blood mononuclear cells (PBMC) of patients with ovarian neoplasia. PBMC from 13 volunteer patients with ovarian neoplasia, seven benign and six malignant tumours, were cultured in CellGro medium supplemented with anti-CD3 (9-10 initial days), IL-2 and foetal bovine serum for 21 days. The resulting effector cells were evaluated for their phenotype, cytotoxicity and cytokine secretion. PBMC cultures resulted in multiple populations (NK, NKT and T) of effector cells, enriched with CD56(+) lymphocytes. NK cells from patients with benign and malignant ovarian neoplasia were expanded 139.6 ± 63.4 and 82.7 ± 25.3-fold, respectively, being the largest lymphocyte subtype among CD56(+) population. Effector cells expanded from patients with malignant ovarian neoplasia had higher proportion of T lymphocytes and altered cytokine production patterns, characterized by lower INF-γ, TNF-α and higher IL-4, compared with patients with benign ovarian neoplasia. Effector cells were cytotoxic against K562 and OVCAR3 cell lines. Cytotoxicity was significantly higher (P < 0.05) using magnetically separated CD56(+) effector cell fractions compared with CD56-deprived ones. The present study demonstrates the feasibility of the culture system employed to generate effector cells, enriched with CD56(+) lymphocytes, from PBMC of patients with ovarian neoplasia. NK cells were the largest lymphocyte subtype among the CD56(+) population and the main variable among the final effector cell preparation affecting target cell killing.
Subject: Adult
Antibodies, Monoclonal
Antigens, Cd3
Antigens, Cd56
Cells, Cultured
Cytokines
Cytotoxicity, Immunologic
Female
Flow Cytometry
Humans
Killer Cells, Natural
Leukocytes, Mononuclear
Lymphocyte Count
Middle Aged
Natural Killer T-cells
Ovarian Neoplasms
Rights: fechado
Identifier DOI: 10.1111/j.1365-3083.2011.02576.x
Address: http://www.ncbi.nlm.nih.gov/pubmed/21595734
Date Issue: 2011
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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