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Type: Artigo de periódico
Title: The Cholinergic Response Is Increased In Isolated Ileum From Gastroschisis Rat Model.
Author: Régis, Aline Cristina
Rojas-Moscoso, Julio Alejandro
Gonçalves, Frances Lilian Lanhellas
Schmidt, Augusto Frederico
Mónica, Fabíola Zakia
Antunes, Edson
Sbragia, Lourenço
Abstract: Babies with gastroschisis (G) have high morbidity rate and long hospital stay due to bowel hypomotility caused by chronic exposure of the bowel to the amniotic fluid. Our aim was to evaluate the reactivity of isolated ileum in fetal rats selected for experimental gastroschisis. G was surgically created at 18.5 days of gestation (term = 22 days). Concentration-dependent curve to the muscarinic agonist methacholine (1-30 μM) and contractions induced by electrical field stimulation (EFS, 1-16 Hz, 50 V, 1 ms) were carried out in isolated ileum of groups control (C), sham (S) and gastroschisis (G) (n = 30). Protein expression for M(3) was assessed by western blot analysis. The frequency and amplitude of spontaneous contractions were decreased in G (p < 0.001). Methacholine produced concentration-dependent contractions being the maximal response values higher in G (p < 0.01). EFS-induced frequency-dependent contractions showed 1.8 times higher in G as well as an increase of M(3) expression. The frequency and the amplitude of rhythmic contractions were reduced along with an increase in the contraction induced by mucarinic agonist and by EFS in G. These results suggest the occurrence of an adaptative supersensitivity to cholinergic response via increases in the protein expression for M(3) receptor.
Subject: Animals
Disease Models, Animal
In Vitro Techniques
Rats, Sprague-dawley
Receptors, Cholinergic
Citation: Pediatric Surgery International. v. 27, n. 9, p. 1015-9, 2011-Sep.
Rights: fechado
Identifier DOI: 10.1007/s00383-011-2923-3
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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