Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199405
Type: Artigo de periódico
Title: In Vitro And In Vivo Assessment Of The Anti-malarial Activity Of Caesalpinia Pluviosa.
Author: Kayano, Ana Carolina A V
Lopes, Stefanie C P
Bueno, Fernanda G
Cabral, Elaine C
Souza-Neiras, Wanessa C
Yamauchi, Lucy M
Foglio, Mary A
Eberlin, Marcos N
Mello, João Carlos P
Costa, Fabio T M
Abstract: To overcome the problem of increasing drug resistance, traditional medicines are an important source for potential new anti-malarials. Caesalpinia pluviosa, commonly named sibipiruna, originates from Brazil and possess multiple therapeutic properties, including anti-malarial activity. Crude extract (CE) was obtained from stem bark by purification using different solvents, resulting in seven fractions. An MTT assay was performed to evaluate cytotoxicity in MCF-7 cells. The CE and its fractions were tested in vitro against chloroquine-sensitive (3D7) and -resistant (S20) strains of Plasmodium falciparum and in vivo in Plasmodium chabaudi-infected mice. In vitro interaction with artesunate and the active C. pluviosa fractions was assessed, and mass spectrometry analyses were conducted. At non-toxic concentrations, the 100% ethanolic (F4) and 50% methanolic (F5) fractions possessed significant anti-malarial activity against both 3D7 and S20 strains. Drug interaction assays with artesunate showed a synergistic interaction with the F4. Four days of treatment with this fraction significantly inhibited parasitaemia in mice in a dose-dependent manner. Mass spectrometry analyses revealed the presence of an ion corresponding to m/z 303.0450, suggesting the presence of quercetin. However, a second set of analyses, with a quercetin standard, showed distinct ions of m/z 137 and 153. The findings show that the F4 fraction of C. pluviosa exhibits anti-malarial activity in vitro at non-toxic concentrations, which was potentiated in the presence of artesunate. Moreover, this anti-malarial activity was also sustained in vivo after treatment of infected mice. Finally, mass spectrometry analyses suggest that a new compound, most likely an isomer of quercetin, is responsible for the anti-malarial activity of the F4.
Subject: Animals
Antimalarials
Artemisinins
Brazil
Caesalpinia
Cell Line
Cell Survival
Disease Models, Animal
Drug Synergism
Humans
Malaria
Mice
Mice, Inbred C57bl
Plant Bark
Plant Extracts
Plants, Medicinal
Plasmodium Chabaudi
Plasmodium Falciparum
Quercetin
Rodent Diseases
Citation: Malaria Journal. v. 10, p. 112, 2011.
Rights: aberto
Identifier DOI: 10.1186/1475-2875-10-112
Address: http://www.ncbi.nlm.nih.gov/pubmed/21535894
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_21535894.pdf7.45 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.