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Type: Artigo de periódico
Title: In Vitro And In Vivo Assessment Of The Anti-malarial Activity Of Caesalpinia Pluviosa.
Author: Kayano, Ana Carolina A V
Lopes, Stefanie C P
Bueno, Fernanda G
Cabral, Elaine C
Souza-Neiras, Wanessa C
Yamauchi, Lucy M
Foglio, Mary A
Eberlin, Marcos N
Mello, João Carlos P
Costa, Fabio T M
Abstract: To overcome the problem of increasing drug resistance, traditional medicines are an important source for potential new anti-malarials. Caesalpinia pluviosa, commonly named sibipiruna, originates from Brazil and possess multiple therapeutic properties, including anti-malarial activity. Crude extract (CE) was obtained from stem bark by purification using different solvents, resulting in seven fractions. An MTT assay was performed to evaluate cytotoxicity in MCF-7 cells. The CE and its fractions were tested in vitro against chloroquine-sensitive (3D7) and -resistant (S20) strains of Plasmodium falciparum and in vivo in Plasmodium chabaudi-infected mice. In vitro interaction with artesunate and the active C. pluviosa fractions was assessed, and mass spectrometry analyses were conducted. At non-toxic concentrations, the 100% ethanolic (F4) and 50% methanolic (F5) fractions possessed significant anti-malarial activity against both 3D7 and S20 strains. Drug interaction assays with artesunate showed a synergistic interaction with the F4. Four days of treatment with this fraction significantly inhibited parasitaemia in mice in a dose-dependent manner. Mass spectrometry analyses revealed the presence of an ion corresponding to m/z 303.0450, suggesting the presence of quercetin. However, a second set of analyses, with a quercetin standard, showed distinct ions of m/z 137 and 153. The findings show that the F4 fraction of C. pluviosa exhibits anti-malarial activity in vitro at non-toxic concentrations, which was potentiated in the presence of artesunate. Moreover, this anti-malarial activity was also sustained in vivo after treatment of infected mice. Finally, mass spectrometry analyses suggest that a new compound, most likely an isomer of quercetin, is responsible for the anti-malarial activity of the F4.
Subject: Animals
Cell Line
Cell Survival
Disease Models, Animal
Drug Synergism
Mice, Inbred C57bl
Plant Bark
Plant Extracts
Plants, Medicinal
Plasmodium Chabaudi
Plasmodium Falciparum
Rodent Diseases
Citation: Malaria Journal. v. 10, p. 112, 2011.
Rights: aberto
Identifier DOI: 10.1186/1475-2875-10-112
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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