Please use this identifier to cite or link to this item:
Type: Artigo de periódico
Title: The Influence Of Low Oxygen On Macrophage Response To Leishmania Infection.
Author: Degrossoli, A
Arrais-Silva, W W
Colhone, M C
Gadelha, F R
Joazeiro, P P
Giorgio, S
Abstract: Hypoxia (low oxygen tension) is a common feature of inflamed and infected tissues. The influence of hypoxia on macrophage responses to micro-organisms has only recently been studied. This study demonstrates that hypoxia induced macrophages to control Leishmania amazonensis, an intracellular parasite that causes cutaneous and cutaneous metastatic lesions. The mechanisms that contribute to the control of macrophages against L. amazonensis infection under a hypoxic microenvironment are not known. Nitric oxide, TNF-α, IL-10 or IL-12 is not responsible for the decrease in parasitism under hypoxia. Live L. amazonensis entry or exocytosis of internalized particles as well as energetic metabolism was not impaired in infected macrophages; no apoptosis-like death was detected in intracellular parasites. Reactive oxygen species (ROS) is likely to be involved, because treatment with antioxidants N-acetylcysteine (NAC) and ebselen inhibits the leishmanicidal effect of macrophages under hypoxia. Leishmania amazonensis infection induces macrophages to express hypoxia-inducible factor-1 (HIF-1α) and -2 (HIF-2α). Data indicate that hypoxia affects the microbial activities and protein expression of macrophages leading to a different phenotype from that of the normoxic counterpart and that it plays a role in modulating Leishmania infection.
Subject: Acetylcysteine
Basic Helix-loop-helix Transcription Factors
Free Radical Scavengers
Hypoxia-inducible Factor 1, Alpha Subunit
Leishmaniasis, Cutaneous
Mice, Inbred Balb C
Mice, Inbred C57bl
Mice, Knockout
Nitric Oxide
Nitric Oxide Synthase Type Ii
Organoselenium Compounds
Reactive Oxygen Species
Tumor Necrosis Factor-alpha
Rights: fechado
Identifier DOI: 10.1111/j.1365-3083.2011.02566.x
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_21517930.pdf389.18 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.