Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199345
Type: Artigo de periódico
Title: Fetal Rhd Genotyping By Analysis Of Maternal Plasma In A Mixed Population.
Author: Amaral, Daphne R T
Credidio, Débora C
Pellegrino, Jordão
Castilho, Lilian
Abstract: Maternal plasma analysis for the determination of the fetal RHD status is an exciting tool for the management of RhD-negative pregnant women, specially sensitized women. We assessed the accuracy of fetal RHD genotyping by analysis of maternal plasma in a multi-ethnic population. We analyzed plasma samples from 88 RhD-negative pregnant women between 11 and 39 weeks of gestation, median age of 28 years old to determine the fetal RHD genotype. This population was from Southeastern Brazil with high mixed ethnic background. Fourteen patients (16%) had anti-D alloantibody. We used Taqman primers and probes to detect by real-time PCR, exons 4, 5, and 10 of RHD. As internal controls we used primers/probes sets to SRY and CCR5. Peripheral or umbilical cord bloods from respective neonates were collected during delivery and hemagglutination was performed. Fifty-eight samples (66%) were genotyped as RHD+, 27 samples (31%) showed complete absence of RHD and 3 samples (3 %) presented a D variant (RHDψ). All the results agreed with the neonatal typing, including the three fetuses with the RHDψ, phenotyped as RhD-negative. Thus, the accuracy of the fetal RHD genotyping in this mixed population was 100%. The earliest pregnancy in which fetal RHD was detected was 11 weeks. Our findings indicate that the accuracy of RHD gene using three regions (exons 4, 5, and 10) can be sufficient for clinical application in a multi-ethnic population. This knowledge helped us on the development of a feasible protocol for fetal RHD genotyping on DNA from maternal plasma for our population.
Subject: Adolescent
Adult
Brazil
Dna
Ethnic Groups
Female
Fetal Blood
Genetic Testing
Gestational Age
Humans
Middle Aged
Polymerase Chain Reaction
Pregnancy
Prenatal Diagnosis
Rh-hr Blood-group System
Young Adult
Rights: fechado
Identifier DOI: 10.1002/jcla.20440
Address: http://www.ncbi.nlm.nih.gov/pubmed/21438001
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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