Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199271
Type: Artigo de periódico
Title: Exercise Training Enhances Rat Pancreatic Islets Anaplerotic Enzymes Content Despite Reduced Insulin Secretion.
Author: Zoppi, Claudio C
Calegari, Vivian C
Silveira, Leonardo R
Carneiro, Everardo M
Boschero, Antonio C
Abstract: Endurance exercise has been shown to reduce pancreatic islets glucose-stimulated insulin secretion (GSIS). Anaplerotic/cataplerotic pathways are directly related to GSIS signaling. However, the effect of endurance training upon pancreatic islets anaplerotic enzymes is still unknown. In this sense, we tested the hypothesis that endurance exercise decreases GSIS by reducing anaplerotic/cataplerotic enzymes content. Male Wistar rats were randomly assigned to one of the four experimental groups as follows: control sedentary group (CTL), trained 1 day per week (TRE1×), trained 3 days per week (TRE3×) and trained 5 days per week (TRE5x) and submitted to an 8 weeks endurance-training protocol. After the training protocol, pancreatic islets were isolated and incubated with basal (2.8 mM) and stimulating (16.7 mM) glucose concentrations for GSIS measurement by radioimmunoassay. In addition, pyruvate carboxylase (PYC), pyruvate dehydrogenase (PDH), pyruvate dehydrogenase kinase 4 (PDK4), ATP-citrate lyase (ACL) and glutamate dehydrogenase (GDH) content were quantified by western blotting. Our data showed that 8 weeks of chronic endurance exercise reduced GSIS by 50% in a dose-response manner according to weekly exercise frequency. PYC showed significant twofold increase in TRE3×. PYC enhancement was even higher in TRE5× (p < 0.0001). PDH and PDK4 reached significant 25 and 50% enhancement, respectively compared with CTL. ACL and GDH also reported significant 50 and 75% increase, respectively. The absence of exercise-induced correlations among GSIS and anaplerotic/cataplerotic enzymes suggests that exercise may control insulin release by activating other signaling pathways. The observed anaplerotic and cataplerotic enzymes enhancement might be related to β-cell surviving rather than insulin secretion.
Subject: Atp Citrate (pro-s)-lyase
Animals
Enzymes
Glucose
Glutamate Dehydrogenase
Insulin
Islets Of Langerhans
Male
Physical Conditioning, Animal
Physical Endurance
Protein Kinases
Pyruvate Carboxylase
Rats
Rats, Wistar
Secretory Pathway
Up-regulation
Rights: fechado
Identifier DOI: 10.1007/s00421-011-1842-2
Address: http://www.ncbi.nlm.nih.gov/pubmed/21287194
Date Issue: 2011
Appears in Collections:Unicamp - Artigos e Outros Documentos

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