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Type: Artigo de periódico
Title: Evaluation Of Sample Preparation Protocols For Proteomic Analysis Of Sunflower Leaves.
Author: da Silva, Marcelo Anselmo Oseas
Garcia, Jerusa Simone
de Souza, Gustavo Henrique Martins Ferreira
Eberlin, Marcos Nogueira
Gozzo, Fábio César
Arruda, Marco Aurélio Zezzi
Abstract: Six protocols for extraction of proteins from sunflower (Helianthus annuus L.) leaves were evaluated for their abilities in both removing interferents and attaining the best resolution in two-dimensional gel electrophoresis. Classical phenol extraction followed by precipitation with ammonium acetate in methanol displayed the most efficient protocol, which allowed the detection of 244 protein spots with ca. 485mug of protein in gel electrophoresis. Tandem mass spectrometry was performed to identify proteins in 61 spots, and cross species identification was used for this task. Proteins from twenty two spots were identified, and 12 of these proteins are up to now not included into the ExPASy sunflower protein databank.
Subject: Amino Acid Sequence
Databases, Protein
Electrophoresis, Gel, Two-dimensional
Electrophoresis, Polyacrylamide Gel
Plant Leaves
Plant Proteins
Tandem Mass Spectrometry
Citation: Talanta. v. 80, n. 4, p. 1545-51, 2010-Feb.
Rights: fechado
Identifier DOI: 10.1016/j.talanta.2009.06.016
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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