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Type: Artigo de periódico
Title: Immediate Response Of Myocardium To Pressure Overload Includes Transient Regulation Of Genes Associated With Mitochondrial Bioenergetics And Calcium Availability.
Author: Deckmann, Ana Carolina
Theizen, Thaís Holz
Medrano, Francisco Javier
Franchini, Kleber Gomes
Pereira, Gonçalo Amarante Guimarães
Abstract: Ventricular hypertrophy is one of the major myocardial responses to pressure overload (PO). Most studies on early myocardial response focus on the days or even weeks after induction of hypertrophic stimuli. Since mechanotransduction pathways are immediately activated in hearts undergoing increased work load, it is reasonable to infer that the myocardial gene program may be regulated in the first few hours. In the present study, we monitored the expression of some genes previously described in the context of myocardial hypertrophic growth by using the Northern blot technique, to estimate the mRNA content of selected genes in rat myocardium for the periods 1, 3, 6, 12 and 48 h after PO stimuli. Results revealed an immediate switch in the expression of genes encoding alpha and beta isoforms of myosin heavy chain, and up-regulation of the cardiac isoform of alpha actin. We also detected transitory gene regulation as the increase in mitochondrial cytochrome c oxidase 1 gene expression, parallel to down-regulation of genes encoding sarco(endo)plasmic reticulum Ca(+2) ATPase and sodium-calcium exchanger. Taken together, these results indicate that initial myocardial responses to increased work load include alterations in the contractile properties of sarcomeres and transitory adjustment of mitochondrial bioenergetics and calcium availability.
Subject: Serca2
Gene Expression
Myocardial Hypertrophy
Pressure Overload
Citation: Genetics And Molecular Biology. v. 33, n. 1, p. 12-6, 2010-Jan.
Rights: aberto
Identifier DOI: 10.1590/S1415-47572010005000004
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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