Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199145
Type: Artigo de periódico
Title: Molecular Characterization Of G And P-types Bovine Rotavirus Strains From Goiás, Brazil: High Frequency Of Mixed P-type Infections.
Author: Caruzo, Thabata Alessandra Ramos
Brito, Willia Marta Elsner Diederichsen de
Munford, Veridiana
Rácz, Maria Lúcia
Abstract: In this study, 331 samples from calves less than one month old from a dairy herd in the district of Piracanjuba, state of Goiás, Brazil were tested for rotavirus. Thirty-three samples (9.9%) tested positive for rotavirus. Out of those, 31 were submitted to G and P characterization by reverse transcription followed by semi-nested polymerase chain reaction. Two samples were characterized as G6P[1], three as G10P[11] and five as G6P[11]. The majority of the samples (51.6%) displayed multiple P genotypes (P-genotype mixtures), including typical human genotypes P[4] and P[6M], suggesting the occurrence of co-infections and genetic reassortment. Also, the detection of human genotypes in bovine samples may be considered evidence of the zoonotic potential of rotaviruses. To our knowledge, this is the first report of such a high frequency of P genotype mixtures in bovine rotavirus samples. It also increases data on G and P rotavirus genotypes circulating in dairy herds in Brazil and can help in the development of more efficient immunization approaches, thereby controlling infection and reducing economical losses.
Subject: Animals
Brazil
Cattle
Cattle Diseases
Electrophoresis, Polyacrylamide Gel
Feces
Genotype
Humans
Polymerase Chain Reaction
Rna, Viral
Reverse Transcriptase Polymerase Chain Reaction
Rotavirus
Rotavirus Infections
Rights: aberto
Identifier DOI: 
Address: http://www.ncbi.nlm.nih.gov/pubmed/21225202
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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