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Type: Artigo de periódico
Title: Characterization Of Hnek6 Interactome Reveals An Important Role For Its Short N-terminal Domain And Colocalization With Proteins At The Centrosome.
Author: Vaz Meirelles, Gabriela
Ferreira Lanza, Daniel Carlos
da Silva, Júlio César
Santana Bernachi, Jéssica
Paes Leme, Adriana Franco
Kobarg, Jörg
Abstract: Physical protein-protein interactions are fundamental to all biological processes and are organized in complex networks. One branch of the kinome network is the evolutionarily conserved NIMA-related serine/threonine kinases (Neks). Most of the 11 mammalian Neks studied so far are related to cell cycle regulation, and due to association with diverse human pathologies, Neks are promising chemotherapeutic targets. Human Nek6 was associated to carcinogenesis, but its interacting partners and signaling pathways remain elusive. Here we introduce hNek6 as a highly connected member in the human kinase interactome. In a more global context, we performed a broad data bank comparison based on degree distribution analysis and found that the human kinome is enriched in hubs. Our networks include a broad set of novel hNek6 interactors as identified by our yeast two-hybrid screens classified into 18 functional categories. All of the tested interactions were confirmed, and the majority of tested substrates were phosphorylated in vitro by hNek6. Notably, we found that hNek6 N-terminal is important to mediate the interactions with its partners. Some novel interactors also colocalized with hNek6 and γ-tubulin in human cells, pointing to a possible centrosomal interaction. The interacting proteins link hNek6 to novel pathways, for example, Notch signaling and actin cytoskeleton regulation, or give new insights on how hNek6 may regulate previously proposed pathways such as cell cycle regulation, DNA repair response, and NF-κB signaling. Our findings open new perspectives in the study of hNek6 role in cancer by analyzing its novel interactions in specific pathways in tumor cells, which may provide important implications for drug design and cancer therapy.
Subject: Binding Sites
Carrier Proteins
Cell Line, Tumor
Hek293 Cells
Microscopy, Confocal
Models, Biological
Protein Binding
Protein-serine-threonine Kinases
Signal Transduction
Two-hybrid System Techniques
Citation: Journal Of Proteome Research. v. 9, n. 12, p. 6298-316, 2010-Dec.
Rights: fechado
Identifier DOI: 10.1021/pr100562w
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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