Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199021
Type: Artigo de periódico
Title: Evidence Of Chagas Disease In Seronegative Brazilian Patients With Megaesophagus.
Author: Batista, Angelica M
Aguiar, Camila
Almeida, Eros A
Guariento, Maria E
Wanderley, Jamiro S
Costa, Sandra C B
Abstract: After 100 years of research, Chagas disease (CD) remains an important public health problem in Latin America. The symptomatic chronic phase is usually characterized by cardiac or digestive involvement and diagnosis currently relies on the measurement of Trypanosoma cruzi-specific antibodies produced in response to the infection. However, the detection of parasite DNA in seronegative persons has been reported. The prevalence of CD in a population with esophageal disorders was assessed by conventional serology. We also detected T. cruzi DNA in blood samples of seronegative and inconclusive patients by nested polymerase chain reaction (N-PCR). The seroprevalence of CD determined by conventional serologic tests (indirect immunofluorescence (IIF) and enzyme-linked immunosorbent assay (ELISA)) was 79% in 513 patients with esophageal disorders. Out of 41 blood samples, N-PCR was positive in 31 (76%) cases for which serology was negative or inconclusive. As all patients presented with clinical signs suggestive of the digestive form of CD and most of them were born in endemic areas, we highlight the importance of improving diagnosis of the disease and the implications for blood bank screening. Our data suggest that N-PCR is effective in the detection of T. cruzi DNA in patients with inconclusive or negative serology, and it may eventually be useful in the determination of the etiology of megaesophagus.
Subject: Adult
Aged
Aged, 80 And Over
Brazil
Chagas Disease
Dna, Protozoan
Enzyme-linked Immunosorbent Assay
Esophageal Achalasia
Female
Fluorescent Antibody Technique, Indirect
Humans
Latin America
Male
Middle Aged
Polymerase Chain Reaction
Prevalence
Retrospective Studies
Seroepidemiologic Studies
Trypanosoma Cruzi
Young Adult
Rights: fechado
Identifier DOI: 10.1016/j.ijid.2010.05.017
Address: http://www.ncbi.nlm.nih.gov/pubmed/20833571
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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