Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/199001
Type: Artigo de periódico
Title: Changes In Cell Migration-related Molecules Expressed By Thymic Microenvironment During Experimental Plasmodium Berghei Infection: Consequences On Thymocyte Development.
Author: Gameiro, Jacy
Nagib, Patrícia R A
Andrade, Carolina F
Villa-Verde, Déa M S
Silva-Barbosa, Suse D
Savino, Wilson
Costa, Fábio T M
Verinaud, Liana
Abstract: We previously showed alterations in the thymus during experimental infection with Plasmodium berghei. Such alterations comprised histological changes, with loss of cortical-medullary limits, and the intrathymic presence of parasites. As the combination of chemokines, adhesion molecules and extracellular matrix (ECM) is critical to appropriate thymocyte development, we analysed the thymic expression of ECM ligands and receptors, as well as chemokines and their respective receptors during the experimental P. berghei infection. Increased expression of ECM components was observed in thymi from infected mice. In contrast, down-regulated surface expression of fibronectin and laminin receptors was observed in thymocytes from these animals. Moreover, in thymi from infected mice there was increased CXCL12 and CXCR4, and a decreased expression of CCL25 and CCR9. An altered thymocyte migration towards ECM elements and chemokines was seen when the thymi from infected mice were analysed. Evaluation of ex vivo migration patterns of CD4/CD8-defined thymocyte subpopulations revealed that double-negative (DN), and CD4(+) and CD8(+) single-positive (SP) cells from P. berghei-infected mice have higher migratory responses compared with controls. Interestingly, increased numbers of DN and SP subpopulations were found in the spleens of infected mice. Overall, we show that the thymic atrophy observed in P. berghei-infected mice is accompanied by thymic microenvironmental changes that comprise altered expression of thymocyte migration-related molecules of the ECM and chemokine protein families, which in turn can alter the thymocyte migration pattern. These thymic disturbances may have consequences for the control of the immune response against this protozoan.
Subject: Animals
Antigens, Cd4
Antigens, Cd8
Cell Movement
Cells, Cultured
Chemokines
Gene Expression Regulation
Malaria
Male
Mice
Mice, Inbred Balb C
Models, Animal
Plasmodium Berghei
Precursor Cells, T-lymphoid
Receptors, Cytoadhesin
Receptors, Fibronectin
Receptors, Laminin
Thymus Gland
Citation: Immunology. v. 129, n. 2, p. 248-56, 2010-Feb.
Rights: fechado
Identifier DOI: 10.1111/j.1365-2567.2009.03177.x
Address: http://www.ncbi.nlm.nih.gov/pubmed/19824923
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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