Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198990
Type: Artigo de periódico
Title: Renal Kinetics Of Bothrops Alternatus (urutu) Snake Venom In Rats.
Author: Mello, Sueli M
Linardi, Alessandra
Rennó, André L
Tarsitano, Christiane A B
Pereira, Elionai M
Hyslop, Stephen
Abstract: The renal kinetics of Bothrops alternatus venom (0.8 mg/kg, i.v.) was studied in conscious male Wistar rats. Blood, urine and renal tissue samples were collected at various intervals after envenoming. Venom was quantified by ELISA in serum, renal tissue and urine. Urine volume was measured and the urine assayed for urobilinogen, glucose, bilirubin, ketones, urine specific gravity, occult blood, pH, protein, nitrite and leucocytes. Circulating venom showed biexponential kinetics, with no venom being detected after 7 days post-venom. Venom was detected in renal tissue 30 min post-venom but decreased progressively thereafter, in parallel with serum venom concentrations. Immunohistochemistry detected venom in glomeruli, proximal and distal tubules, and vascular and perivascular tissue. Venom was detected in urine 3, 6 and 24 h post-venom. Oliguria occurred 3 h to 7 days post-venom, urine acidification occurred 3-6 h post-venom, urine specific gravity increased in the first 3 h and proteinuria was also greatest in this period. Creatinine clearance decreased progressively until 24-48 h post-venom, then returned to normal. Glucose, ketones, leucocytes and occult blood were detected mainly during the first 6 h post-venom. These results indicate reversible alterations in renal function, with renal elimination of the venom.
Subject: Acute Kidney Injury
Animals
Antivenins
Bothrops
Creatinine
Crotalid Venoms
Electrophoresis, Polyacrylamide Gel
Enzyme-linked Immunosorbent Assay
Immunohistochemistry
Indicators And Reagents
Kidney
Male
Rats
Rats, Wistar
Rights: fechado
Identifier DOI: 10.1016/j.toxicon.2009.09.018
Address: http://www.ncbi.nlm.nih.gov/pubmed/19818360
Date Issue: -1-Uns- -1
Appears in Collections:Unicamp - Artigos e Outros Documentos

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