Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198960
Type: Artigo de periódico
Title: Involvement Of Regulatory T Cells In The Immunosuppression Characteristic Of Patients With Paracoccidioidomycosis.
Author: Ferreira, Maria Carolina
de Oliveira, Rômulo Tadeu Dias
da Silva, Rosiane Maria
Blotta, Maria Heloisa Souza Lima
Mamoni, Ronei Luciano
Abstract: Patients with paracoccidioidomycosis (PCM) exhibit a suppression of the cellular immune response characterized by negative delayed-type hypersensitivity (DTH) to Paracoccidioides brasiliensis antigens, the apoptosis of lymphocytes, and high levels of expression of cytotoxic-T-lymphocyte-associated antigen 4 (CTLA-4), interleukin-10 (IL-10), and transforming growth factor β (TGF-β). The aim of this study was to investigate whether and how regulatory T cells (Treg cells) are involved in this immunosuppression by analyzing the number, phenotype, and activity of these cells in patients with active disease (AD group) and patients who had received treatment (TD group). Our results showed that the AD patients had more Treg cells than the TD patients or controls (C group) and also had elevated levels of expression of regulatory markers (glucocorticoid-induced tumor necrosis factor [TNF] receptor-related protein [GITR], CTLA-4, CD95L, LAP-1, and CD38). An analysis of regulatory activity showed that Treg cells from the AD group had greater activity than did cells from the other groups and that cell-cell contact is mandatory for this activity in the C group but was only partially involved in the regulatory activity of cells from AD patients. The addition of anti-IL-10 and anti-TGF-β neutralizing antibodies to the cultures showed that the production of cytokines may be another mechanism used by Treg cells. In conclusion, the elevated numbers of these cells with an increased regulatory phenotype and strong suppressive activity suggest a potential role for them in the immunosuppression characteristic of paracoccidioidomycosis. In addition, our results indicate that while Treg cells act by cell-cell contact, cytokine production also plays an important role.
Subject: Antifungal Agents
Coculture Techniques
Cytokines
Gene Expression Regulation
Humans
Immunity, Cellular
Immunocompromised Host
Paracoccidioidomycosis
Rna, Messenger
T-lymphocyte Subsets
T-lymphocytes, Regulatory
Rights: aberto
Identifier DOI: 10.1128/IAI.00487-10
Address: http://www.ncbi.nlm.nih.gov/pubmed/20643858
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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