Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198937
Type: Artigo de periódico
Title: Purification And Characterization Of A New Weak Hemorrhagic Metalloproteinase Bmhf-1 From Bothrops Marajoensis Snake Venom.
Author: Torres-Huaco, Frank Denis
Ponce-Soto, Luis Alberto
Martins-de-Souza, Daniel
Marangoni, Sergio
Abstract: BmHF-1, from the venom of Bothrops marajoensis, was purified by Sephadex G-75 and HPLC-RP on micro-Bondapak C-18 column chromatography. It presented a molecular mass of 27162.36 Da determined by MALDI-TOF MS. BmHF-1 had a sequence of 238 residues of amino acids. The multiple alignment of its amino acid sequence and those of other snake venom metalloproteinases showed high structural similarity, mainly among P-I class. The enzyme initially cleaves the Aalpha-chain of fibrinogen, followed by the Bbeta-chain, and shows no effects on the gamma-chain. BmHF-1 had, caseinolytic and weakly hemorrhagic activities, which were inhibited by EDTA. In contrast, PMSF did not affect these activities. The caseinolytic activity of BmHF-1 had a pH optimum of 8.0 and was stable in solution up to 40 degrees C; activity was completely lost at > or = 70 degrees C. The proteolytic activity was also inhibited by sDa (opossum sera) and Da2-1, Da2-II, antihemorrhagic factors isolated from the opossum sera of Didelphis albiventris. BmHF-1 presents weak hemorrhagic activity, with a MHD of 41.14 microg and it induces dose-dependent edema. We could concluded that, despite its weak hemorrhagic activity, BmHF-1 contributes to local tissue damage by inducing edema, releasing pharmacologically active mediators from protein precursors due to its enzymatic action.
Subject: Amino Acid Sequence
Analysis Of Variance
Animals
Bothrops
Chromatography, Gel
Chromatography, High Pressure Liquid
Chromatography, Reverse-phase
Crotalid Venoms
Fibrinogen
Hemorrhage
Male
Metalloproteases
Mice
Molecular Sequence Data
Sequence Alignment
Sequence Analysis, Protein
Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization
Temperament
Rights: fechado
Identifier DOI: 10.1007/s10930-010-9267-z
Address: http://www.ncbi.nlm.nih.gov/pubmed/20607373
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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