Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198929
Type: Artigo de periódico
Title: Preparation And Characterization Of Ethanol-treated Silk Fibroin Dense Membranes For Biomaterials Application Using Waste Silk Fibers As Raw Material.
Author: Nogueira, Grínia M
Rodas, Andrea C D
Leite, Carlos A P
Giles, Carlos
Higa, Olga Z
Polakiewicz, Bronislaw
Beppu, Marisa M
Abstract: The possibility of producing valued devices from low cost natural resources is a subject of broad interest. The present study explores the preparation and characterization of silk fibroin dense membranes using waste silk fibers from textile processing. Morphology, crystallinity, thermal resistance and cytotoxicity of membranes as well as the changes on the secondary structure of silk fibroin were analyzed after undergoing treatment with ethanol. Membranes presented amorphous patterns as determined via X-ray diffraction. The secondary structure of silk fibroin on dense membranes was either random coil (silk I) or beta-sheet (silk II), before and after ethanol treatment, respectively. The sterilized membranes presented no cytotoxicity to endothelial cells during in vitro assays. This fact stresses the material potential to be used in the fabrication of biomaterials, as coatings of cardiovascular devices and as membranes for wound dressing or drug delivery systems.
Subject: Animals
Biocompatible Materials
Biotechnology
Cho Cells
Calorimetry, Differential Scanning
Cell Proliferation
Cricetinae
Cricetulus
Endothelial Cells
Ethanol
Fibroins
Humans
Mechanical Phenomena
Membranes, Artificial
Spectroscopy, Fourier Transform Infrared
Temperature
Tensile Strength
Thermogravimetry
Waste Products
X-ray Diffraction
Rights: fechado
Identifier DOI: 10.1016/j.biortech.2010.06.064
Address: http://www.ncbi.nlm.nih.gov/pubmed/20598877
Date Issue: 2010
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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