Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198927
Type: Artigo de periódico
Title: Taurine Supplementation: Involvement Of Cholinergic/phospholipase C And Protein Kinase A Pathways In Potentiation Of Insulin Secretion And Ca2+ Handling In Mouse Pancreatic Islets.
Author: Ribeiro, Rosane A
Vanzela, Emerielle C
Oliveira, Camila A M
Bonfleur, Maria L
Boschero, Antonio C
Carneiro, Everardo M
Abstract: Taurine (TAU) supplementation increases insulin secretion in response to high glucose concentrations in rodent islets. This effect is probably due to an increase in Ca2+ handling by the islet cells. Here, we investigated the possible involvement of the cholinergic/phospholipase C (PLC) and protein kinase (PK) A pathways in this process. Adult mice were fed with 2% TAU in drinking water for 30 d. The mice were killed and pancreatic islets isolated by the collagenase method. Islets from TAU-supplemented mice showed higher insulin secretion in the presence of 8.3 mm-glucose, 100 μm-carbachol (Cch) and 1 mm-3-isobutyl-1-methyl-xanthine (IBMX), respectively. The increase in insulin secretion in response to Cch in TAU islets was accompanied by a higher intracellular Ca2+ mobilisation and PLCβ2 protein expression. The Ca2+ uptake was higher in TAU islets in the presence of 8.3 mm-glucose, but similar when the islets were challenged by glucose plus IBMX. TAU islets also showed an increase in the expression of PKAα protein. This protein may play a role in cation accumulation, since the amount of Ca2+ in these islets was significantly reduced by the PKA inhibitors: N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinoline sulfonamide (H89) and PK inhibitor-(6-22)-amide (PKI). In conclusion, TAU supplementation increases insulin secretion in response to glucose, favouring both influx and internal mobilisation of Ca2+, and these effects seem to involve the activation of both PLC-inositol-1,4,5-trisphosphate and cAMP-PKA pathways.
Subject: 1-methyl-3-isobutylxanthine
Animals
Calcium
Carbachol
Cells, Cultured
Colforsin
Cyclic Amp-dependent Protein Kinases
Cytoplasm
Dietary Supplements
Insulin
Islets Of Langerhans
Mice
Phorbol Esters
Phospholipase C Beta
Protein Kinase C-alpha
Taurine
Rights: fechado
Identifier DOI: 10.1017/S0007114510001820
Address: http://www.ncbi.nlm.nih.gov/pubmed/20591207
Date Issue: 2010
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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