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Type: Artigo de periódico
Title: Antenatal Steroid And Tracheal Occlusion Restore Vascular Endothelial Growth Factor Receptors In Congenital Diaphragmatic Hernia Rat Model.
Author: Schmidt, Augusto F
Gonçalves, Frances L L
Nassr, Azize C C
Pereira, Luis A V D
Farmer, Diana
Sbragia, Lourenço
Abstract: Investigate the effects of antenatal steroids and tracheal occlusion on pulmonary expression of vascular endothelial growth factor receptors in rats with nitrofen-induced congenital diaphragmatic hernia. Fetuses were exposed to nitrofen at embryonic day 9.5. Subgroups received dexamethasone or were operated on for tracheal occlusion, or received combined treatment. Morphologic variables were recorded. To analyze vascular endothelial growth factor receptor 1 and vascular endothelial growth factor receptor 2 expression, we performed Western blotting and immunohistochemistry. Morphologic variables were analyzed by analysis of variance and immunohistochemistry by Kruskal-Wallis test. Congenital diaphragmatic hernia decreased body weight, total lung weight, and lung-to-body weight ratio. Tracheal occlusion increased total lung weight and lung-to-body weight ratio (P < .05). Fetuses with congenital diaphragmatic hernia had reduced vascular endothelial growth factor receptor 1 and vascular endothelial growth factor receptor 2 expression, whereas steroids and tracheal occlusion increased their expression. Combined treatment increased expression of receptors, but had no additive effect. Vascular endothelial growth factor signaling disruption may be associated with pulmonary hypertension in congenital diaphragmatic hernia. Tracheal occlusion and steroids provide a pathway for restoring expression of vascular endothelial growth factor receptors.
Subject: Animals
Blotting, Western
Disease Models, Animal
Hernia, Diaphragmatic
Hernias, Diaphragmatic, Congenital
Maternal Exposure
Phenyl Ethers
Rats, Sprague-dawley
Receptors, Vascular Endothelial Growth Factor
Sensitivity And Specificity
Tracheal Stenosis
Citation: American Journal Of Obstetrics And Gynecology. v. 203, n. 2, p. 184.e13-20, 2010-Aug.
Rights: fechado
Identifier DOI: 10.1016/j.ajog.2010.04.022
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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