Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198854
Type: Artigo de periódico
Title: Purification Of Fructooligosaccharides In An Activated Charcoal Fixed Bed Column.
Author: Kuhn, Raquel Cristine
Filho, Francisco Maugeri
Abstract: Fructooligosaccharides (FOS) are mixtures of oligosaccharides containing mono and disaccharides, therefore, the separation of these sugars results in purer products for human consumption and can be added to various food products (drinks, cookies and yogurt). The aim of this work was the purification of fructooligosaccharides from a mixture of sugars, obtained by enzymatic synthesis, containing fructooligosaccharides, glucose, fructose and sucrose using activated charcoal fixed bed column. Temperature and ethanol concentration effects were analyzed using a 2² central composite design. Good separation conditions were obtained through central composite design. The best separation coefficient between fructooligosaccharides and glucose (ES(fructoolig/gluc)) was 3.99 ± 0.07 and between fructose and fructooligosaccharides (ES(fructoolig/fruct)) was 2.89 ± 0.36 using ethanol 15% (v/v) as eluent, at 50°C. The final FOS purification degree and recovery were about 80% and 97.8%, respectively. The activated charcoal fixed bed columns were shown to be a good alternative for sugar separation, especially for rich mixtures of fructooligosaccharides.
Subject: Adsorption
Carbohydrates
Charcoal
Chromatography
Functional Food
Humans
Molecular Structure
Oligosaccharides
Rights: fechado
Identifier DOI: 10.1016/j.nbt.2010.05.008
Address: http://www.ncbi.nlm.nih.gov/pubmed/20478421
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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