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Type: Artigo de periódico
Title: Protocol On Induction Of Tmj Articular Disc Degeneration In Rats By Utilization Of Botulinum Toxin.
Author: Da Ré Guerra, Flávia
de Souza Pires, Ivan Luiz
de Aro, Andrea Aparecida
Camargo, Lilian Calili
Pimentel, Edson Rosa
Palomari, Evanisi Teresa
Abstract: To develop a model of experimental degeneration in the articular disc of the TMJ of rats through the use of botulinum toxin that can be used in future studies of degenerative diseases on fibrocartilage. Aiming at the above-mentioned objective, 12 Lewis male rats were used and divided into two groups: CG, control group and DG, group of animals to which the botulinum toxin was administered (6 units/kg). The morphological analysis was carried out utilizing histological cuts stained with hematoxyline-eosine, toluidine blue and Picrosirius; the biochemical analysis was made by SDS-polyacrylamide gel electrophoresis. The DG showed peculiar characteristics regarding a degeneration joint disk, compatible with those described in literature as: reduction of cells number, general disorganization of cells direction and extracellular matrix, increase in glycosaminoglycans content and degradation of the tissue collagen. Based on the morphological and biochemical results, it was concluded that the proposed degeneration model showed to be satisfactory for futures studies of injuries and fibrocartilage regeneration processes.
Subject: Animals
Botulinum Toxins, Type A
Cartilage Diseases
Cell Count
Collagen Type I
Coloring Agents
Disease Models, Animal
Electrophoresis, Polyacrylamide Gel
Extracellular Matrix
Extracellular Matrix Proteins
Fluorescent Dyes
Injections, Intramuscular
Masseter Muscle
Neuromuscular Agents
Rats, Inbred Lew
Temporomandibular Joint Disc
Temporomandibular Joint Disorders
Rights: fechado
Identifier DOI: 10.1016/j.archoralbio.2010.03.018
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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